目的 :建立血浆中美洛昔康浓度的高效液相色谱分析方法 ,用此方法对大鼠灌胃给药 ,对 3种剂量的美洛昔康进行药动学研究。方法 :色谱条件 :色谱柱为C18(4.6mm× 15 0mm ,5 μm) ,流动相为甲醇 水 冰醋酸 乙腈 (6 0 0∶5 0 0∶2 0∶5 0 ) ,流速1.0ml.min-1,检测波长 35 5nm ,柱温 4 0℃ ,血药浓度 时间数据采用PKBP N1程序拟合其药动学参数。结果 :血浆中内源性杂质不干扰美洛昔康及其分解产物的测定 ,血浆中回收率为 98.80 % ,RSD为 1.78%。日间RSD <7.17% ,日内RSD <6 .6 9%。大鼠灌胃给药 1.0 ,2 .0 ,6 .0mg·kg-1美洛昔康后 ,血药浓度时间曲线符合二室开放模式 ,并由此测得美洛昔康在大鼠体内的半衰期分别为 4 3.2 5 ,38.6 0 ,37.10h ,达峰时间分别为 2 .0 2 ,1.90 ,1.94h。峰浓度分别为 2 .5 2 ,4 .85 ,10 .6 2 μg·ml-1,AUC分别为 112 .8,14 2 .2 ,4 12 .0 μg·h·ml-1。结论 :本方法简便 ,准确 ,能较好地满足美洛昔康血药浓度监测及药动学的要求 OBJECTIVE: To establish a HPLC method for the determination of meloxicam in plasma. The method was used for intragastric administration of meloxicam in rats. The pharmacokinetics of three doses of meloxicam were studied. Methods: Chromatographic conditions were as follows: C18 (4.6 mm × 150 mm, 5 μm) column with methanol-water glacial acetic acid (60:50:50:20:50) at a flow rate of 1.0 ml · min- 1, detection wavelength 35 5nm, column temperature 4 0 ℃, plasma concentration time data using PKBP N1 program to fit their pharmacokinetic parameters. Results: Plasma endogenous impurities did not interfere with the determination of meloxicam and its decomposition products. The plasma recovery was 98.80% and the RSD was 1.78%. Daytime RSD <7.17%, day RSD <6.96%. After intragastric administration of 1.0, 2.0, 6.0 mg · kg-1 meloxicam in rats, the time curve of plasma concentration conforms to the two-compartment open pattern, and thus meloxicam can be measured in rats The half-lives were 4 3.2 5, 38.6 0 and 37.10 h, respectively. The peak times were 2.00 2, 1.90 and 1.94 h, respectively. The peak concentrations were 2.52, 4.85 and 10.62 μg · ml-1, respectively, and the AUCs were 112.8,14.2 and 42.01 μg · h · ml-1, respectively. Conclusion: The method is simple and accurate, and can meet the requirements of meloxicam plasma concentration monitoring and pharmacokinetics