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为探究中国和美国大豆疫霉的遗传关系,采用随机扩增DNA多态性(RAPD)的方法,对来自中国黑龙江省、福建省和美国的3个大豆疫霉地理群体的遗传多样性进行了分析.通过使用21个RAPD引物对供试的111株大豆疫霉菌株进行扩增,共得到223个RAPD标记,其中多态性标记为199个,占89.23%.遗传变异分析表明,美国群体具有更高的遗传变异度;Nei’s遗传相似性和主成分分析均显示,中国福建群体与美国群体间的遗传相似性最高,而福建群体与黑龙江群体间遗传相似性最低;聚类分析显示,供试菌株在88%的相似性水平上可被区分为7个聚类,且美国群体分布于更多的聚类组中;Shannon-Wiener多样性指数也表明美国群体的遗传多样性最为丰富.综合分析表明,本研究的结果不支持关于美国的大豆疫霉可能来源于中国的推测.
In order to explore the genetic relationship between Phytophthora sojae in China and the United States, the genetic diversity of three Phytophthora sojae populations from Heilongjiang Province, Fujian Province and the United States of China was investigated using random amplified polymorphic DNA (RAPD) A total of 223 RAPD markers were amplified by using 21 RAPD primers, of which 199 were polymorphic markers, accounting for 89.23%. Genetic variation analysis showed that the United States population had Higher genetic variation; Nei’s genetic similarity and principal component analysis showed that the genetic similarity between Fujian and the United States population was the highest in Fujian, while the genetic similarity was the lowest in Fujian and Heilongjiang. Cluster analysis showed that the test The strains could be divided into 7 clusters at 88% similarity level and the United States population was distributed in more cluster groups. The Shannon-Wiener diversity index also showed that the United States population had the most abundant genetic diversity. This suggests that the results of this study do not support the speculation that P. aeruginosa may originate in China.