目的:建立测定地龙注射液中主要氨基酸含量的方法。方法:以6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯(AQC)为衍生剂,与地龙注射液中的游离氨基酸柱前定量衍生,用HPLC法测定衍生物含量。结果:丙氨酸、缬氨酸、盐酸赖氨酸、异亮氨酸和亮氨酸在各自浓度范围内线性关系良好(r≥0.999 8),平均回收率102.6%～104.9%,RSD 0.3%～0.9%。结论:本法简便、准确,可用于地龙注射液的生产和质量控制。 Objective: To establish a method for determination of the main amino acids in Dilong injection. Methods: 6-Aminoquinoline-N-hydroxysuccinimidyl carbamate (AQC) as a derivatizing agent was used to quantitatively derive the free amino acid precursor from Digong Injection. The content of the derivative was determined by HPLC. Results: The linear relationship between alanine, valine, lysine hydrochloride, isoleucine and leucine was good (r≥0.999 8), the average recovery was 102.6% -104.9%, RSD was 0.3% ~ 0.9%. Conclusion: This method is simple and accurate and can be used for the production and quality control of Dilong injection.