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目的了解浙江省二级生物安全(BSL-2)实验室生物安全柜年检情况,为规范生物安全实验室管理提供依据。方法对浙江省各级医疗机构、疾病预防控制机构、科研院所、大专院校等机构登记的1 019个实验室的生物安全柜年检情况进行分析,并比较不同机构、地区生物安全柜年检情况。结果共登记生物安全柜1166台,其中未到检测年检67台,占5.75%;超过检测期限后年检105台,占9.00%;定期年检64台,占5.49%;超过检测期限仍未年检930台,占79.76%。医疗机构、疾病预防控制机构、其他机构(科研院所、大专院校等)生物安全柜定期年检的比例分别为5.26%、3.68%和14.29%,而超期未年检的比例分别为80.19%、88.10%和43.96%,不同机构生物安全柜年检情况差异有统计学意义(P<0.05)。浙江省各市生物安全柜定期年检比例为0.00%~33.33%,超期未年检比例为26.67%~94.12%,其中超期未年检比例≥85.00%的有温州、宁波、衢州和嘉兴4个市。结论全省大多数BSL-2实验室的生物安全柜未能及时年检,存在一定的生物安全隐患。
Objective To understand the annual inspection of BSL-2 laboratory biological safety cabinets in Zhejiang Province and provide the basis for standardizing the management of biosafety laboratories. Methods The annual inspection of biological safety cabinets in 1 019 laboratories registered by medical institutions, disease prevention and control institutions, scientific research institutes and tertiary institutions in Zhejiang Province was analyzed. The annual inspection of biological safety cabinets in different institutions and regions was compared . Results A total of 1166 biological safety cabinets were registered, of which 67 were not yet tested for annual inspection, accounting for 5.75%; 105 were annually inspected after the inspection period was over, accounting for 9.00%; 64 were regularly examined annually, accounting for 5.49%; 930 units were still not examined annually , Accounting for 79.76%. The proportion of regular annual inspection of biological safety cabinets in medical institutions, disease prevention and control institutions and other institutions (scientific research institutes, tertiary institutions, etc.) was 5.26%, 3.68% and 14.29% respectively, while the proportion of non-annual inspection overdue was 80.19% and 88.10 respectively % And 43.96% respectively. There were significant differences in annual inspection of biological safety cabinets among different institutions (P <0.05). Zhejiang Province, the city’s biological safety cabinets regular annual inspection rate of 0.00% to 33.33%, the expiry of the annual inspection rate was 26.67% ~ 94.12%, of which overdue annual inspection rate ≥ 85.00% of Wenzhou, Ningbo, Quzhou and Jiaxing 4 cities. Conclusion Most of the BSL-2 laboratories in the province failed to conduct annual biosafety cabinets inspection in a timely manner, which posed a potential biosafety hazard.