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目的探讨中药气血宁对心肌梗死大鼠心脏结构、功能及ghrelin表达的影响。方法将雄性SD大鼠随机分为假手术组、模型组、倍他乐克组、气血宁小剂量组和气血宁大剂量组。采用结扎大鼠心脏左冠状动脉前降支的方法制作心肌梗死大鼠模型,假手术组平行手术操作但不结扎前降支。术后灌胃给药4周。采用超声心动图检测心脏结构、功能,HE染色观察心肌组织病理,实时荧光定量PCR检测ghrelin及其受体GHSR mRNA的表达。结果与假手术组比较,模型组、倍他乐克组、气血宁小剂量组和气血宁大剂量组收缩末期的室间隔厚度均显著变薄(P<0.05,P<0.01),左室内径显著增大(P<0.01),左室射血分数和左室短轴缩短分数明显降低(P<0.01);模型组舒张末期的左室内径显著增大(P<0.05),心肌组织ghrelin及其受体GHSR mRNA的表达显著增高(P<0.01)。与模型组比较,气血宁大剂量组左室射血分数和左室短轴缩短分数明显增大(P<0.05);倍他乐克组、气血宁小剂量组和气血宁大剂量组的心肌组织病理损伤有所改善,ghrelin及其受体GHSR mRNA的表达显著降低(P<0.05,P<0.01)。结论中药气血宁能部分改善心肌梗死大鼠心脏结构、功能,调节ghrelin及其受体GHSR mRNA的表达。
Objective To investigate the effect of qiuxuening on cardiac structure, function and ghrelin expression in myocardial infarction rats. Methods Male Sprague-Dawley rats were randomly divided into sham operation group, model group, Betaloc group, Qixuening low-dose group and Quxuening high-dose group. A rat model of myocardial infarction was established by ligation of the left anterior descending artery of the rat heart. The sham operation group was operated in parallel but without descending coronary artery. After oral administration for 4 weeks. Cardiac structure and function were detected by echocardiography. The histopathology of myocardium was observed by HE staining. The expression of ghrelin and its receptor GHSR mRNA was detected by real-time fluorescence quantitative PCR. Results Compared with the sham operation group, the thickness of the interventricular septum in the model group, metoprolol, Quxuening low-dose group and qiuxuening high-dose group was significantly thinner (P <0.05, P <0.01), left (P <0.01), left ventricular ejection fraction and left ventricular fractional shortening fraction decreased significantly (P <0.01). The left ventricular diameter at end-diastolic phase in model group was significantly increased (P <0.05) Ghrelin and its receptor GHSR mRNA expression was significantly increased (P <0.01). Compared with the model group, the QLXR group had a significant increase of left ventricular ejection fraction and shortening of left ventricular fraction (P <0.05); Betaloc, QXRH and QNR Myocardial histopathological injury was improved, ghrelin and its receptor GHSR mRNA expression was significantly reduced (P <0.05, P <0.01). Conclusion QHYX could partly improve the cardiac structure and function of myocardial infarction rats and regulate the expression of ghrelin and its receptor GHSR mRNA.