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目的在具有免疫能力的BALB/c小鼠上构建播散性B淋巴瘤动物模型。方法经尾静脉注射鼠源性B细胞淋巴瘤细胞株A20于同源BALB/c小鼠,0~3个月间观察动物成瘤情况;取动物脏器行石蜡包埋、病理切片、HE染色;流式细胞仪检测其CD19的表达。结果尾静脉注射2×106、2×107细胞于14只BALB/c小鼠,成瘤时间分别为76.8±12.0天和26.1±7.9天;总体成瘤率分别为71.4%(5/7)和100%(7/7);在肝脏、脾脏、淋巴结、肠、肠系膜、下肢、颈部、子宫和臀部等多脏器成瘤;流式检测瘤组织细胞mCD19表达强阳性。结论运用尾静脉注射方法构建了内脏器官广泛发生的播散性B淋巴瘤BALB/c小鼠动物模型,为进一步进行B淋巴瘤相关研究提供了实验依据。
OBJECTIVE: To construct an animal model of disseminated B lymphoma on immunocompetent BALB / c mice. Methods The mice were injected with tail vein-derived murine B cell lymphoma cell line A20 in homologous BALB / c mice for 0 ~ 3 months to observe the tumorigenesis of the animals. The animals were paraffin-embedded, histopathological sections and HE staining The expression of CD19 was detected by flow cytometry. Results The tumorigenic time of 2 × 106 and 2 × 107 cells in 14 BALB / c mice was 76.8 ± 12.0 days and 26.1 ± 7.9 days, respectively. The total tumorigenic rates were 71.4% (5/7) and 100% (7/7); tumorigenicity in multiple organs such as liver, spleen, lymph node, intestine, mesentery, lower extremity, neck, uterus and buttock; Conclusion The animal model of BALB / c mice with disseminated B lymphoma, which is widely occurring in internal organs, was constructed by tail vein injection and provided experimental evidence for the further study of B lymphoma.