论文部分内容阅读
采用高效液相色谱法测定小儿清咽冲剂中牛蒡苷的含量。色谱柱:Agilent ExtendODS C18;流动相:乙腈—水(23∶77);流速:1.0ml.min-1;检测波长:280nm;进样量:20μl。牛蒡苷在25~250μg.ml-1浓度范围内,峰面积积分值与浓度呈良好的线性关系(r=0.9999);平均回收率为100.03%,RSD=0.1%(n=6)。本方法简便、准确、重现性好。
Determination of abierosporin in Xiaoer Qingyan Granules by high performance liquid chromatography. Column: Agilent ExtendODS C18; mobile phase: acetonitrile-water (23:77); flow rate: 1.0 ml.min-1; detection wavelength: 280 nm; injection volume: 20 μl. In the concentration range of 25-250 μg.ml-1, there was a good linear relationship between the peak area integral and concentration (r=0.9999). The average recovery rate was 100.03% and RSD was 0.1% (n=6). This method is simple, accurate and reproducible.