论文部分内容阅读
目的探讨不同剂量氨基葡萄糖(GS)对实验性自身免疫性脑脊髓炎(EAE)大鼠血-脑脊液屏障(BBB)功能及动态变化的影响。方法用完全抗原和减毒百日咳杆菌原液免疫大鼠后随机分为3组:①EAE组(n=40):腹腔注射4.5(ml/kg·d)磷酸盐缓冲溶液。②GS治疗1组(n=40):腹腔注射90mg/(kg·d)GS溶液。③GS治疗2组(n=40):腹腔注射180mg/(kg·d)GS溶液。各组分别于免疫后6、8、10、12、14、16、18d检测大鼠血清和脑脊液(CSF)中白蛋白(ALB)含量,其CSF与血清ALB比值(QA)作为评价BBB功能及动态变化的指标。结果EAE组QA值在免疫后6d开始升高,免疫后10d达最大值,早于临床症状出现。GS治疗组QA值在免疫后6d仅有少量升高,在免疫后14d达高峰,与EAE组相比QA值变化趋势差异有统计学意义(P<0.01)。GS治疗2组QA值与GS治疗1组相比有一定程度的降低。结论GS可降低EAE大鼠QA值水平,对EAE发病具有一定程度的保护作用。
Objective To investigate the effects of different doses of glucosamine (GS) on the function and dynamic changes of blood-brain barrier (BBB) in experimental autoimmune encephalomyelitis (EAE) rats. Methods The rats were immunized with whole antigen and attenuated Bordetella pertussis. The rats were randomly divided into 3 groups: ①EAE group (n = 40): intraperitoneal injection of 4.5 (ml / kg · d) phosphate buffered saline. GS treatment group 1 (n = 40): intraperitoneal injection of 90mg / (kg · d) GS solution. ③ GS treatment group 2 (n = 40): intraperitoneal injection of 180mg / (kg · d) GS solution. The levels of albumin (ALB) in serum and cerebrospinal fluid (CSF) were measured at 6, 8, 10, 12, 14, 16 and 18 days after immunization. The ratio of CSF to serum ALB was used to evaluate BBB function and Dynamic indicators of change. Results The QA value of EAE group began to increase at 6 days after immunization, reaching the maximum at 10 days after immunization, earlier than the clinical symptoms. The QA value of GS treatment group increased only slightly at 6 days after immunization and peaked at 14 days after immunization. There was significant difference in QA value between EAE group and GSE group (P <0.01). GS treatment group 2 QA value and GS treatment group 1 compared to a certain degree of reduction. Conclusion GS can reduce the level of QA in EAE rats and protect the development of EAE to a certain extent.