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目的:应用基因表达谱芯片研究HCV非结构蛋白NS5A反式激活基因NS5ATP13的反式调节基因. 方法:构建NS5ATP13基因的真核表达载体pcDNA3.1(-)- NS5ATP13,应用基因表达谱芯片技术对pcDNA3.1(-)- NS5ATP13转染的人肝母细胞瘤细胞系HepG2细胞和转染空载体的相同细胞的差异表达mRNA进行检测和分析. 结果:HepG2细胞经转染NS5ATP13后,有86条差异基因表达,其中46条基因表达增强,40条基因表达降低.这些差异表达的基因与细胞的增生、分化及细胞的信号转导、代谢、凋亡密切相关. 结论:应用基因表达谱芯片成功筛选了NS5ATP13的反式调节基因,为进一步阐明NS5ATP13的反式激活作用及免疫调节机制提供了新的依据.
Objective: To study the trans-regulatory gene of NS5A transactivator NS5A, a nonstructural protein NS5A, by gene expression microarray.Methods: The eukaryotic expression vector pcDNA3.1 (-) - NS5ATP13 of NS5ATP13 gene was constructed and gene expression profiling pcDNA3.1 (-) - NS5ATP13 transfected human hepatoblastoma cell line HepG2 cells transfected with empty vector and the same cells were detected by mRNA expression and analysis.Results: HepG2 cells transfected with NS5ATP13, 86 Differentially expressed genes, of which 46 genes were enhanced and 40 genes were decreased.These differentially expressed genes were closely related to cell proliferation, differentiation and cell signal transduction, metabolism and apoptosis.Conclusion: The trans-regulation gene of NS5ATP13 was screened, which provided a new basis for further elucidating the transactivation and immunomodulatory mechanism of NS5ATP13.