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目的:从氧化损伤角度,探讨芎芪合剂抗溶栓后脑缺血再灌注损伤的作用机理。方法:建立SD大鼠大脑中动脉闭塞(MCAO)及溶栓模型,模拟溶栓成功后的再灌注。运用病理TTC染色、组织生化检测和Northern b lot杂交的方法,进行脑梗死面积测定、抗氧化损伤相关指标(清道夫受体CD36基因表达、SOD、MDA、GSH-PX、NOS、iNOS含量)测定。结果:(1)芎芪合剂能够使SD大鼠缺血再灌注侧脑组织梗死面积明显缩小。(2)芎芪合剂能抑制缺血再灌注引起的清道夫受体CD36 mRNA表达增高;芎芪合剂能够调节缺血再灌注后SOD、MDA、GSH-PX、NOS、iNOS的含量,并使之趋向于正常。结论:(1)芎芪合剂具有抗脑缺血再灌注损伤的作用;(2)芎芪合剂能够通过减轻氧化应激损伤的程度达到抗脑缺血再灌注损伤的作用。
OBJECTIVE: To investigate the anti-thrombotic effects of cerebral reperfusion injury after thrombolytic therapy from the perspective of oxidative damage. METHODS: Middle cerebral artery occlusion (MCAO) and thrombolysis models were established in SD rats to simulate reperfusion after successful thrombolysis. Pathological TTC staining, tissue biochemical detection, and Northern b lot hybridization were used to determine the cerebral infarction area and the related indicators of anti-oxidative damage (the expression of the scavenger receptor CD36 gene, SOD, MDA, GSH-PX, NOS, and iNOS). . RESULTS: (1) Sputum mixture could significantly reduce the infarct size of cerebral ischemia-reperfusion side of SD rats. (2) The chelator can inhibit the expression of scavenger receptor CD36 mRNA induced by ischemia-reperfusion; the chelator can regulate the content of SOD, MDA, GSH-PX, NOS, iNOS after ischemia-reperfusion, and make it Tends to be normal. Conclusion: (1) The mixture of peony has anti-cerebral ischemia-reperfusion injury. (2) The mixture of lanthanum can reduce the degree of oxidative damage to achieve the effect of anti-cerebral ischemia-reperfusion injury.