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[目的]利用凋亡相关基因芯片研究苦参碱对K562细胞基因表达谱的影响,以揭示苦参碱抗肿瘤作用的分子机制。[方法]苦参碱0.4mg/ml作用K562细胞48h后,分别提取对照组和处理组细胞总RNA,采用Superarray公司GEArrayQSeriesHumanApoptosisGeneArray(HS-002)芯片(含96个凋亡相关基因)筛选出苦参碱作用细胞后的差异表达基因,并用RT-PCR验证其中的LIGHT基因表达的变化。[结果]0.4mg/ml苦参碱作用K562细胞48h后,芯片结果显示37个基因的差异表达在2倍以上,其中表达下调为32个,表达上调为5个。RT-PCR验证了苦参碱对LIGHT基因表达上调的影响。[结论]苦参碱作用K562细胞可上调LIGHT基因的表达。
[Objective] To study the effect of matrine on the gene expression profile of K562 cells by using apoptosis-related gene microarray to reveal the molecular mechanism of matrine’s anti-tumor effect. [Method] K562 cells were treated with matrine 0.4 mg / ml for 48 h, and the total RNA was extracted from the control group and the treated group, respectively. Sophora flavescens (Sophora flavescens Mill.) Was selected by using Superarray GEArrayQSeriesHumanApoptosisGeneArray (HS-002) Alkaline-induced cells were differentially expressed, and the changes of LIGHT gene expression were verified by RT-PCR. [Results] After treated with 0.4mg / ml matrine for 48h, the result of chip showed that the expression of 37 genes was more than 2 times, and the expression was down-regulated to 32, and the expression was up-regulated to 5. The effect of matrine on the up-regulation of LIGHT gene expression was verified by RT-PCR. [Conclusion] Matrine can up-regulate the expression of LIGHT gene in K562 cells.