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目的研究茯苓多糖(PCP)中PCP-Ⅰ和PCP-Ⅱ作为疫苗佐剂的免疫原性。方法 1采用钥孔戚血蓝蛋白(KLH)和牛血清白蛋白(BSA)为载体蛋白分别与PCP-Ⅰ或PCP-Ⅱ连接制备免疫抗原KLHPCP-Ⅰ和KLH-PCP-Ⅱ及筛选抗原BSA-PCP-Ⅰ和BSA-PCP-Ⅱ。KLH-PCP-Ⅰ和KLH-PCP-Ⅱ分别与弗氏佐剂联用id免疫家兔2次,ELISA检测家兔血清中抗多糖抗体。2PCP-Ⅰ或PCP-Ⅱ单独im免疫小鼠2次,ELISA检测小鼠血清中抗多糖抗体。3PCP-Ⅰ或PCP-Ⅱ为佐剂分别配伍重组乙肝病毒表面抗原(HBs Ag)和猪繁殖与呼吸综合征病毒灭活疫苗(PRRSV)im或sc免疫小鼠2次,ELISA检测小鼠血清中抗多糖抗体。结果1KLH-PCP-Ⅰ或KLH-PCP-Ⅱ与弗氏佐剂联用免疫2次,可产生抗KLH和抗多糖抗体。2PCP-Ⅰ或PCP-Ⅱ单独im免疫小鼠2次,检测出低水平Ig M抗体,未检测出IgG抗体。3HBs Ag或PRRSV抗原联用PCP-Ⅰ或PCP-Ⅱ免疫小鼠2次,未检出抗多糖IgG抗体。结论 PCP-Ⅰ和PCP-Ⅱ本身免疫原性较弱,作为疫苗佐剂可能具有良好的安全性。
Objective To study the immunogenicity of PCP-Ⅰ and PCP-Ⅱ as vaccine adjuvants in tuckahoe polysaccharide (PCP). Method 1 KLHPCP-Ⅰ and KLH-PCP-Ⅱ were prepared by using keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA) as carrier protein and PCP-Ⅰ or PCP-Ⅱ to screen BSA-PCP -I and BSA-PCP-II. KLH-PCP-I and KLH-PCP-Ⅱ were immunized twice with Freund’s adjuvant with id, and anti-polysaccharide antibody was detected by ELISA. The mice were immunized twice with 2PCP-I or PCP-II, and the anti-polysaccharide antibodies in serum were detected by ELISA. 3PCP-Ⅰ or PCP-Ⅱ were adjuvants were combined with recombinant hepatitis B virus surface antigen (HBs Ag) and porcine reproductive and respiratory syndrome virus inactivated vaccine (PRRSV) im or sc were immunized mice 2 times, ELISA detection of serum in mice Anti-polysaccharide antibody. Results 1KLH-PCP-Ⅰ or KLH-PCP-Ⅱ immunized with Freund’s adjuvant twice, can produce anti-KLH and anti-polysaccharide antibodies. Mice were immunized twice with 2PCP-I or PCP-II im to detect low-level IgM antibodies and no IgG antibodies were detected. 3HBs Ag or PRRSV antigens were immunized twice with PCP-I or PCP-II, and anti-polysaccharide IgG antibody was not detected. Conclusion PCP-Ⅰ and PCP-Ⅱ are weak in their own immunogenicity and may have good safety as a vaccine adjuvant.