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作者选用经过修饰、高度减毒的复制缺陷型痘苗病毒 MVA株为载体 ,构建了含有呼吸道合胞病毒 (RSV) F和 /或 G糖蛋白基因的重组体 ,分别为 MVA/ RSV F、MVA/RSV G、MVA/ RSV FG。各 MVA重组体都能在鸡胚成纤维细胞中很好繁殖。表达 F或G蛋白的单重组病毒的滴度比表达两种蛋白的
The authors selected a modified and highly attenuated replication-deficient vaccinia virus MVA strain as a vector to construct a recombinant containing the respiratory syncytial virus (RSV) F and / or G glycoprotein genes, MVA / RSV F, MVA / RSV G, MVA / RSV FG. Each MVA recombinant can reproduce well in chicken embryo fibroblasts. The titer of the single recombinant virus expressing the F or G protein is more than that of the two proteins