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我们利用新疆在宰马季节冰冻存放的马脾脏,经过对几种提纯方法的比较,提取和纯化了马脾铁蛋白,并在车间进行了生产。提纯的铁蛋白通过聚丙烯酰胺凝胶电泳鉴定,与进口的马脾铁蛋白一致,均由三条电泳带组成。含铁量约占铁蛋白的20~23%左右,镉的含量低于进口样品。提纯的铁蛋白与兔抗马脾铁蛋白免疫血清之间呈现单一的沉淀线。
We use horse spleen stored frozen in the Sama season in Xinjiang, after several purification methods, extraction and purification of horse spleen ferritin, and in the workshop for the production. The purified ferritin was identified by polyacrylamide gel electrophoresis and was identical to the imported horse spleen ferritin, and consisted of three bands. Iron content of about 20 to 23% of ferritin, cadmium content lower than imported samples. Purified ferritin and rabbit anti-horse spleen ferritin immune serum showed a single precipitation line.