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目的 :探讨重组人肿瘤坏死因子(rhTNF)引起肺癌细胞(LC 6)死亡的机制。方法 :应用原位细胞毒性分析、DNA降解片段琼脂糖凝胶电泳、形态学和流式细胞学测定的动态分析。结果 :rhTNF作用于肺癌细胞(LC 6)数小时后 ,引起细胞核DNA解成寡聚核苷酸片断 ,说明肺癌细胞(LC 6)以细胞凋亡的方式死亡 ,并且细胞凋亡的程度与重组人肿瘤坏死因子作用的时间及浓度呈正相关。单纯应用放线菌素D后则肺癌细胞(LC 6)表现为细胞坏死 ,rhTNF与放线菌素D伍用后可以明显增强这种作用。结论 :rhTNF是通过激发LC 6细胞发生细胞凋亡而发挥其细胞毒性作用。
Objective: To investigate the mechanism of death of lung cancer cells (LC 6) induced by recombinant human tumor necrosis factor (rhTNF). Methods : Dynamic analysis using in situ cytotoxicity analysis, DNA fragmentation agarose gel electrophoresis, morphology and flow cytometry. RESULTS: A few hours after the action of rhTNF on lung cancer cells (LC 6) resulted in the cleavage of nuclear DNA into oligonucleotide fragments, indicating that lung cancer cells (LC 6) died as apoptotic cells, and the extent and reorganization of apoptosis. The time and concentration of human tumor necrosis factor were positively correlated. After the application of actinomycin D alone, the lung cancer cells (LC 6) exhibited cell necrosis, and the effect of rhTNF and actinomycin D could be significantly enhanced. CONCLUSION : rhTNF exerts its cytotoxic effect by stimulating apoptosis in LC 6 cells.