目的和方法 :应用Na+、K+选择性微电极检测马桑内酯致痫大鼠海马及海马脑片神经细胞外Na+、K+活度的改变。结果 :海马内注射马桑内酯 (5 μL ,5× 10 -4 mol/L)致痫大鼠 30s、1min和 2min后 ,海马神经细胞外Na+活度分别低于对照组 2 7 7mmol/L、5 0 3mmol/L和 5 7 8mmol/L ,而K+活度则分别高于对照组 2 3mmol/L、2 4mmol/L和 2 9mmol/L(P <0 0 1)。 3min后 ,K+活度基本恢复至对照水平 ,而Na+活度仍持续低于对照水平 (P <0 0 1)。海马脑片的实验结果与在体实验相似。结论 :海马神经细胞处于癫痫状态时 ,存在Na+内流、K+外流现象。 PURPOSE AND METHODS: Na + and K + selective microelectrodes were used to detect the changes of Na + and K + activity in the hippocampus and hippocampal slices of epileptic rats induced by. Results: The Na + activity of hippocampal neurons in hippocampus of rats with epilepsy induced by episesulose (5 μL, 5 × 10 -4 mol / L) for 30 s, 1 min and 2 min were lower than that of the control group (277 mmol / L , 5 0 3 mmol / L and 5 7 8 mmol / L respectively, while K + activity was higher than that of the control group 2 3mmol / L, 24mmol / L and 29mmol / L respectively (P <0.01). After 3 minutes, the K + activity returned to the control level, while the Na + activity remained below the control level (P <0.01). The experimental results of hippocampal slices are similar to in vivo experiments. Conclusion: There are Na + influx and K + efflux in hippocampal neurons in epileptic state.