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牙鲆(Paralichthys olivaceus)养殖病害日益严重,迟缓爱德华氏菌(Edwardsiella tarda)是牙鲆养殖中的主要致病菌,带来了极大的经济损失,为了培育出对该病抵抗能力强的牙鲆新品种,2014年4–6月间,利用我们长期以来建立和筛选出来的牙鲆抗病群体和家系的亲鱼,通过杂交,自交,雌核发育建立47个家系,9–10月间,对其中39个家系进行感染实验,先用少量鱼进行预实验摸索出半致死浓度,然后再进行正式感染实验,感染数量为每个家系80尾。各家系的存活率范围为1.19%~51.19%,最终平均存活率为20.29%,认为存活率高于30%的7个家系为抗病力强家系,存活率在20.29%~30%的9个家系为抗病力一般家系,平均存活率以下的23个家系为抗病力弱家系。1406#家系抗病力最强,它为1005#家系的自交后代,而其他抗病力强家系也大多是1005#、09104#、0915#的后代。而09104#为0768#的后代,并且0768#抗鳗弧菌病能力强。1005#为韩国群体自交后代,0915#为韩国群体和日本群体杂交后代。这些抗病力强的牙鲆可以作为新品系进行推广养殖,可望减少牙鲆腹水病发生。
Paralichthys olivaceus breeding diseases are increasingly serious, slow Edwardsiella (Edwardsiella tarda) is the main pathogens flounder culture, bringing great economic losses, in order to develop resistance to the disease teeth The new species of flounder, from April to June 2014, used 47 long-term established and screened flounder Paralichthys olivaceus families and their broodstock to establish 47 pedigrees by crossing, selfing and gynogenetic development. From September to October, , Of which 39 families were infected with experiments, first with a small amount of fish pre-experiment to explore the semi-lethal concentration, and then the formal infection experiments, the number of infections per family 80. The survival rate ranged from 1.19% to 51.19% in each family and the final average survival rate was 20.29%. Seven families with survival rates> 30% were resistant families with a survival rate of 20.29% ~ 30% 9 A family of general resistance to the disease family, the average survival rate of the following 23 families for the resistance of weak families. The 1406 # family was the strongest in terms of disease resistance, which is the self-progeny of the 1005 # pedigree, while most of the other disease resistant families are also the offspring of 1005 #, 09104 # and 0915 #. While 09104 # is the offspring of 0768 #, and 0768 # is resistant to Vibrio anguillarum. 1005 # is a self-progeny of Korean population, and 0915 # is a cross of offspring of Korean population and Japanese population. These disease-resistant flounder can be promoted as a new breed breeding, is expected to reduce the occurrence of flounder water disease.