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目的:探讨南蛇藤总萜对荷肝癌HepA1-6小鼠移植瘤生长的影响及其可能机制。方法:建立肝癌HepA1-6小鼠移植瘤模型,分为空白对照组、溶媒对照组(1%DMSO)、阴性对照组(0.9%氯化钠溶液)、阳性对照组(顺铂)及南蛇藤总萜低(10 mg/kg)、中(20 mg/kg)和高(40 mg/kg)剂量组。观察南蛇藤总萜对小鼠体质量、抑瘤率、肝脏指数、脾脏指数及胸腺指数的影响;应用免疫组织化学法检测移植瘤组织中血管内皮生长因子(vascular end othelial growth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的表达以及微血管密度(microvessel density,MVD)计数;TUNEL法检测移植瘤组织中的细胞凋亡。结果:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,促进肿瘤细胞凋亡,下调移植瘤组织中VEGF和bFGF的表达以及MVD计数,与阴性对照组比较,差异有统计学意义(P<0.05);南蛇藤总萜组小鼠肝、脾和胸腺指数及体质量与阴性对照组比较,差异无统计学意义(P>0.05)。结论:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,可能与其促进肿瘤细胞凋亡及抑制肿瘤血管生成有关。
OBJECTIVE: To investigate the effect and possible mechanism of total terpenoids of Celastrus orbiculatus on the growth of hepatocellular carcinoma HepA1-6 transplanted tumor in mice. Methods: HepA1-6 hepatocarcinoma xenografts model was established and divided into blank control group, vehicle control group (1% DMSO), negative control group (0.9% sodium chloride solution), positive control group (cisplatin) Triterpenoids were low (10 mg / kg), medium (20 mg / kg) and high (40 mg / kg). The effects of total terpene of Celastrus orbiculatus on the body weight, tumor inhibition rate, liver index, spleen index and thymus index of mice were observed. The expression of vascular endothelium growth factor (VEGF) in tumor tissue was detected by immunohistochemistry, And basic fibroblast growth factor (bFGF) expression and microvessel density (MVD) were counted. TUNEL assay was used to detect the apoptosis in xenografted tumor tissue. Results: Total antler of Celastrus orbiculatus could significantly inhibit the growth of transplanted hepatoma HepA1-6 mice, promote the apoptosis of tumor cells, downregulate the expression of VEGF and bFGF in tumor tissues and the MVD count. Compared with the negative control group, the difference was (P <0.05). There was no significant difference in the indexes of liver, spleen and thymus and the body weight of mice in the total terpene group (P> 0.05). Conclusion: Total antler of Celastrus orbiculatus can significantly inhibit the growth of transplanted hepatoma HepA1-6 mice, which may be related to the promotion of tumor cell apoptosis and tumor angiogenesis.