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目的 观察完全康复期严重急性呼吸综合征 (SARS)患者外周血针对SARS冠状病毒(SARS CoV)S抗原多肽的特异性细胞免疫反应。方法 分离外周血单个核细胞 (PBMC) ,经混合S抗原多肽刺激后 ,采用酶联免疫吸附试验 (ELISA)和酶联免疫斑点试验 (ELISPOT)检测 ,分析完全康复期SARS患者抗原特异性T淋巴细胞应答反应。结果 当SARS CoV的S抗原混合多肽刺激后 ,只有SARS康复期患者的PBMC分泌大量的IFN γ。同时 ,IFN γ产生细胞的阳性率也显著高于健康对照组 (P <0 .0 5)。此外 ,当用多克隆刺激剂 (PMA +ionomycin)刺激后 ,正常人和康复期SARS患者的PBMC产生等量的IFN γ及IFN γ产生细胞 ,两者经统计学处理 ,差异无统计学意义 (P >0 .0 5)。结论SRAS CoV不仅能诱导中和抗体的产生 ,而且还可诱导抗原特异性细胞免疫应答反应 ,并可在体内长期维持免疫记忆功能。
Objective To observe the specific cellular immune responses to SARS-CoV S-antigen peptide in peripheral blood of patients with complete recovery from severe acute respiratory syndrome (SARS). Methods Peripheral blood mononuclear cells (PBMCs) were isolated and stimulated with mixed S antigen peptides. ELISA and ELISPOT were used to detect the expression of antigen - specific T lymphocytes in patients with complete recovery of SARS. Cell response response. Results When stimulated with the S antigen cocktail of SARS CoV, only PBMC from SARS convalescent patients secrete large amounts of IFNγ. Meanwhile, the positive rate of IFNγ-producing cells was also significantly higher than that of the healthy control group (P <0.05). In addition, PBMC from normal and convalescent SARS patients produced equal amounts of IFNγ and IFNγ-producing cells when stimulated with PMA + ionomycin, both of which were statistically different (P <0.05) P> 0 .0 5). Conclusion SRAS CoV not only induces the production of neutralizing antibodies, but also induces antigen-specific cellular immune responses and maintains long-term immune memory in vivo.