[目的]建立超高效液相色谱-串联质谱(UPLC-MS/MS)检测蔬果中残留分析方法。[方法]采用乙腈提取多杀霉素A、D,UPLC-MS/MS进行定性和定量。[结果]多杀霉素A、D在0.005~0.5 mg/L范围内线性关系良好,相关系数均在0.999 5以上;在0.02~0.5 mg/L添加质量浓度范围内,回收率为72.3%~116.3%,相对标准偏差为1.10%~4.92%;最低检出质量浓度为0.5μg/L。[结论]方法简便、快速、定量准确、灵敏度和精密度好,可实现蔬菜水果中多杀霉素农残分析的要求。 [Objective] To establish a method for the determination of residues in fruits and vegetables by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS). [Method] Acetonitrile was used to extract spinosad A, D and UPLC-MS / MS for qualitative and quantitative analysis. [Result] Spinosyn A and D had a good linearity in the range of 0.005-0.5 mg / L with the correlation coefficients above 0.999 5. The recoveries of spinosad in the range of 0.02-0.5 mg / L were 72.3% 116.3%, the relative standard deviation was 1.10% ~ 4.92%; the lowest detected concentration was 0.5μg / L. [Conclusion] The method was simple, rapid, accurate in quantification, good in sensitivity and precision, and could meet the requirements of pesticide residues analysis of spinosad in vegetables and fruits.