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目的 :复制小鼠高碘甲状腺肿模型 ,研究高蛋白质营养对高碘甲状腺肿的预防作用。方法 :将昆明雄性小鼠随机分成 4组 :H组饮高碘水 (碘浓度为 2 5 0 0 μg/L) ,食普通鼠饲料 ,不含酪蛋白 ;C1和C2 组饮高碘水 ,同时饲养高蛋白饲料 (C1组为鼠饲料混入 2 5 %酪蛋白 ,C2 组混入 5 0 %酪蛋白 ) ;N组为对照组 ,食普通鼠饲料 ,饮自来水 (碘浓度为7 5 μg/L)。实验时间为 15 0天 ,观察小鼠甲状腺重量、甲状腺碘含量 (Riesco碘测定方法 )、甲状腺组织学结构 (光镜和电镜 )、甲状腺和垂体功能及TPO活性 (愈创木酚分析法 )。结果 :H组甲状腺碘含量、血FT4 水平较对照组、C1和C2 组明显升高 (P <0 .0 0 1) ,TPO活性明显下降 (P <0 .0 0 1) ,甲状腺呈典型的胶质性甲状腺肿。结论 :高碘对甲状腺有损伤作用 ,高酪蛋白膳食能明显抑制高碘甲状腺肿的发生 ,机制可能是其阻止过量碘进入甲状腺内。
Objective: To duplicate mouse model of high iodine goiter and to study the preventive effect of high protein nutrition on high iodine goiter. Methods: Kunming male mice were randomly divided into 4 groups: group H, drinking high iodine water (iodine concentration 2550 μg / L), fed normal rat diet without casein; group C1 and C2 drinking high iodine water, At the same time, high-protein feed was fed (C1 group: 25% casein in mouse feed and 50% casein in C2 group); the control group was fed with normal dietary feed and tap water (iodine concentration: 75 μg / L ). The experiment was performed for 15 days. Thyroid weight, thyroid iodine content (Riesco method), thyroid histology (light and electron microscopy), thyroid and pituitary function and TPO activity (guaiacol assay) were observed. Results: The levels of thyroid iodine and blood FT4 in H group were significantly higher than those in control group, C1 and C2 groups (P <0.01), and TPO activity was significantly decreased (P <0.01) Gliosis goitre. Conclusion: High iodine can damage the thyroid gland. The high casein diet can significantly inhibit the occurrence of high iodine goiter. The mechanism may be that it prevents excessive iodine from entering the thyroid gland.