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目的探讨汉族人群中IL-1和TNF-α基因多态性与H.pylori相关性胃十二指肠疾病之间的关系。方法选取H.pylori阳性的142例胃十二指肠疾病患者和140例健康对照者,采用PCR-限制性长度片段多态方法检测该人群中IL-1B-511、TNF-A-308、TNF-A-857位点多态性和IL-1受体拮抗剂基因的多态性。结果 IL-1B-511和IL-1RN各基因型的频率在疾病组和对照组中的分布差异无统计学意义。在疾病组中TNF-A-308和TNF-A-857各基因型的频率与对照组比较,分布有差异,具有统计学意义(P<0.05)。经Logistic回归分析,与携带TNF-A-308G/G者比较,携带TNF-A-308 A/A者发生胃十二指肠疾病的危险性为OR=15.37(95%CI:3.50-67.50);携带TNF-A-308 A/G者发生胃十二指肠疾病的危险性为OR=5.12(95%CI:2.54-10.34);与携带TNF-A-857 C/C者相比较,携带TNF-A-857 T/T者发生胃十二指肠疾病的危险性为OR=3.20(95%CI:1.35-7.60)。结论 IL-1B-511和IL-1RN各基因型与幽门螺杆菌相关性胃十二指肠疾病的发生不相关。TNF-α基因多态性与幽门螺杆菌相关性胃十二指肠疾病的发生相关。
Objective To investigate the relationship between IL-1 and TNF-α gene polymorphisms and H.pylori-related gastroduodenal diseases in Han population. Methods A total of 142 H. pylori-positive patients with gastroduodenal diseases and 140 healthy controls were enrolled in this study. The levels of IL-1B-511, TNF-A-308 and TNF in this population were determined by polymerase chain reaction-restriction fragment length polymorphism -A-857 polymorphism and IL-1 receptor antagonist gene polymorphism. Results The frequencies of IL-1B-511 and IL-1RN genotypes were not significantly different between the disease group and the control group. The frequencies of TNF-A-308 and TNF-A-857 genotypes in the disease group were significantly different from those in the control group (P <0.05). Logistic regression analysis showed that the risk of gastroduodenal diseases with TNF-A-308 A / A was OR = 15.37 (95% CI: 3.50-67.50) compared with TNF-A-308G / (95% CI: 2.54-10.34). Compared with those carrying TNF-A-857 C / C, the risk of carrying gastroduodenal disease with TNF-A-308 A / The risk of gastroduodenal disease in TNF-A-857 T / T patients was OR = 3.20 (95% CI: 1.35-7.60). Conclusion The genotypes of IL-1B-511 and IL-1RN are not related to the occurrence of H. pylori-associated gastroduodenal diseases. TNF-α gene polymorphism and Helicobacter pylori-related gastroduodenal diseases related to the occurrence.