论文部分内容阅读
利用灵芝菌Ganoderma lucidum U-281漆酶对直接蓝86进行酶促氧化脱色,并对其降解机理进行了探讨。结果表明,染料-漆酶共反应体系在20～50℃及pH小于5.0范围内,直接蓝86均可脱色50%以上;漆酶对直接蓝86具有宽泛的浓度适应性,对300 mg/L的该染料仍具有耐受性。最优脱色工艺参数为温度40℃、pH 5.0、染料初始浓度200 mg/L、漆酶用量1 U/mL。在优化条件下,直接蓝863 h的脱色率达到54.54%,48 h脱色率达到91.54%。紫外-可见吸收光谱分析表明,漆酶的酶促氧化导致染料的分子结构产生了变化,是造成直接蓝86脱色的主要发生机制。
Direct blue 86 was enzymatically oxidized and decolorized using Ganoderma lucidum U-281 laccase, and its degradation mechanism was also discussed. The results showed that the dye-laccase co-reaction system could remove more than 50% of the direct blue 86 in the temperature range of 20 ~ 50 ℃ and the pH value was less than 5.0. Laccase had a broad range of adaptability to direct blue 86. The concentration of 300 mg / L The dye is still resistant. The optimum decolorization parameters were temperature 40 ℃, pH 5.0, initial dye concentration 200 mg / L and laccase dosage 1 U / mL. Under optimized conditions, the decolorization rate of direct blue 863 h reached 54.54% and the decolorization rate reached 91.54% at 48 h. UV-Vis absorption spectra showed that the enzymatic oxidation of laccase led to changes in the molecular structure of the dye, which is the main mechanism of decolorization of Direct Blue 86.