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目的探讨微小RNA(mi RNA)在重度子痫前期胎盘组织中的表达水平,为重度子痫患者的治疗机制提供基础。方法选取本院2014年12月-2015年12月收治的重度子痫前期患者8例作为研究,同时选取同期健康体检孕妇者6例进行对比,通过荧光定量PCR检测技术检测胎盘组织中mi RNA-125a-3p、mi RNA-1290以及mi RNA-133b等3个具有代表的mi RNA指标,观察两组表达水平的差异。结果重度子痫患者胎盘组织中mi RNA-125a-3p、mi RNA-1290以及mi RNA-133b表达水平显著高于健康孕妇,差异有统计学意义,P<0.05。结论重度子痫前期患者胎盘组织中mi RNA的表达水平显著高于正常孕妇,其可能参与了重度子痫前期的发病及病理生理过程,说明胎盘组织中mi RNA有可能成为一种新的标记物用于重度子痫的早期诊断。
Objective To investigate the expression of miRNA in the placenta of severe preeclampsia and provide the basis for the treatment of severe eclampsia. Methods Eight patients with severe preeclampsia who were treated in our hospital from December 2014 to December 2015 were enrolled in this study. Six healthy pregnant women in the same period were selected for comparison. MiRNA- 125a-3p, mi RNA-1290 and mi RNA-133b were used to observe the difference of expression level between the two groups. Results The expression levels of mi RNA-125a-3p, mi RNA-1290 and mi RNA-133b in placental tissue of patients with severe eclampsia were significantly higher than those of healthy pregnant women, the difference was statistically significant (P <0.05). Conclusions The expression of mi RNA in placenta of patients with severe preeclampsia is significantly higher than that of normal pregnant women, which may be involved in the pathogenesis and pathophysiology of severe preeclampsia, indicating that mi RNA in placenta may be a new marker For the early diagnosis of severe eclampsia.