目的应用免疫组化方法检测胃腺癌中CD44v6和细胞核因子(NF)-κB的表达特征,探讨二者与临床病理特征的关系及相关性。方法经病理医师确诊的69例胃腺癌患者作为观察组,胃高级别上皮内瘤变患者16例作为对照组1,胃低级别上皮内瘤变患者16例作为对照组2,未见明显异常的胃黏膜组织16例作为对照组3,均留取术后或钳检后的组织,经石蜡包埋后应用免疫组化方法检测CD44v6和NF-κB的表达。结果观察组中CD44v6和NF-κB的阳性率均明显高于对照组1、2和3。观察组中CD44v6和NF-κB的阳性率与淋巴结转移、脉管累犯相关,NF-κB的表达与浸润深度相关。二者均与肿瘤的分化程度无相关性。线性相关分析显示观察组中CD44v6和NF-κB之间无明显相关性。结论胃腺癌中CD44v6和NF-κB表达升高,其可能有利于肿瘤的播散,二者高表达可能预示着不良的生物学行为。 Objective To detect the expression of CD44v6 and NF-κB in gastric adenocarcinoma by immunohistochemistry and to explore the relationship between them and clinicopathological features. Methods Sixty-nine patients with gastric adenocarcinoma diagnosed by pathologist were selected as the observation group, 16 as gastric cancer patients with high grade intraepithelial neoplasia as control group 1, 16 as gastric cancer patients with low grade intraepithelial neoplasia as control group 2, no obvious abnormalities 16 cases of gastric mucosa were taken as control group 3, and the tissues after operation or post-mortem examination were collected. The expressions of CD44v6 and NF-κB were detected by immunohistochemistry after paraffin embedding. Results The positive rates of CD44v6 and NF-κB in observation group were significantly higher than those in control group 1, 2 and 3. The positive rate of CD44v6 and NF-κB in the observation group was correlated with lymph node metastasis and recurrent vessel invasion. The expression of NF-κB was correlated with the depth of invasion. Both had no correlation with the degree of tumor differentiation. Linear correlation analysis showed no significant correlation between CD44v6 and NF-κB in the observation group. Conclusions The expression of CD44v6 and NF-κB in gastric adenocarcinoma is increased, which may contribute to the spread of tumor. The high expression of both may predict the adverse biological behavior.