目的:建立尿砷质控样品的制备方法，并对其均匀性和稳定性进行验证。方法:采集健康成人尿样，浓缩后用冷冻干燥机进行冻干，对冻干后的样品采用原子荧光分光光度法进行砷含量测定。并从均匀性、稳定性、不同检测方法测定、尿砷含量定值等对冻干方法进行验证。结果:方法的标准曲线线性关系相关系数为0.999 7，尿样冻干后砷含量的变异系数均 0.05），样品均匀性良好。稳定性检验结果显示，室温条件下高、低浓度的冻干样品保存时间≤360 d的│下降率│均≤10%。以原子荧光分光光度法和电感耦合等离子体质谱（ICP-MS）法测定高、低浓度冻干尿样中的砷含量，两种方法测定结果比较差异无统计学意义（n P均> 0.05）。14个省级及86个地市县级单位的尿砷含量定值结果，低浓度为（0.028 ± 0.002）mg/L，高浓度为（0.113 ± 0.008）mg/L。n 结论:该制备方法冻干尿砷质控样品，冻干样品的均匀性和稳定性均能够满足于地方病防治监测中实验室的外部质量控制要求，具有可操作性。“,”Objective:To establish a method for preparing urine arsenic quality control samples and verify its uniformity and stability.Methods:Urine samples of healthy adults were collected, concentrated and then freeze-dried using a freeze dryer. The freeze-dried samples were subjected to atomic fluorescence spectrophotometry to determine the arsenic content. The method was verified from the uniformity, stability, determination of different detection methods, and the fixed value of urine arsenic content.Results:The linear correlation coefficient of the standard curve of the method was 0.999 7. The variation coefficients of arsenic content after freeze-dried of urine samples were all 0.05), and the sample uniformity was good. The stability test results show that the decline rate of the freeze-dried samples of high and low concentrations stored ≤360 days was less than 10%, and the stability was good at room temperature. Atomic fluorescence spectrophotometry and inductively coupled plasma mass spectrometry(ICP-MS) were used for the arsenic content determination of high and low concentration samples, and there was no significant difference between the two methods ( n P > 0.05). The results of determination of arsenic content in urine of 14 provinces and 86 cities and counties showed that the low concentration was (0.028 ± 0.002) mg/L and the high concentration was (0.113 ± 0.008) mg/L.n Conclusion:The uniformity and stability of the freeze-dried urine arsenic quality control samples can meet the external quality control requirements of the laboratory in endemic disease prevention and monitoring.