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为了研究一种新的T细胞共刺激分子—CD137对不同状态T细胞增殖和凋亡的双重调节作用。采用3 H TdR掺入法测定T细胞增殖 ,用流式细胞仪测定细胞凋亡。结果显示 :(1)CD137单抗可与T细胞表面的CD137抗原结合 ,明显增强PHA刺激T细胞增殖 ,使T细胞增殖指数较PHA单独作用高 2～ 3倍 ,但CD137单抗单独不能刺激T细胞增殖 ;(2 )对于慢性活化的T细胞 ,CD137单抗可协同PHA诱导T细胞凋亡 ,使T细胞凋亡率从PHA单独作用的 19 2 0 %增加到 36 31% ,CD137单抗单独并不能诱导慢性活化T细胞凋亡。CD137单抗一方面可协同PHA刺激静止状态T细胞的增殖 ,另一方面可协同PHA诱导慢性活化T细胞凋亡 ,对T细胞起双重调节作用。
In order to study the dual regulatory effect of a novel T cell costimulatory molecule-CD137 on proliferation and apoptosis of T cells in different states. T cell proliferation was measured by 3 H TdR incorporation, and apoptosis was measured by flow cytometry. The results showed that: (1) CD137 mAb could bind CD137 antigen on T cells and significantly enhance the proliferation of T cells stimulated by PHA. The proliferation index of T cells was 2 ~ 3 times higher than that of PHA alone. However, CD137 mAb alone could not stimulate T (2) For chronic activated T cells, CD137 mAb can cooperate with PHA to induce T cell apoptosis and increase the rate of T cell apoptosis from 3620% to 3631% of PHA alone, while CD137 mAb alone And can not induce chronic activated T cell apoptosis. On the one hand, CD137 mAb can synergize with PHA to stimulate the proliferation of resting T cells; on the other hand, CD137 can induce the apoptosis of T cells in concert with PHA and play a dual role in regulating T cells.