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研究猕猴多次 sc重组人血小板生成素(rh TPO) 2 μg· kg-1· d-1× 2 0 d或保护剂白蛋白后药代动力学 ,外周血小板计数及血清 TPO抗体变化 ;通过猴血清对 TD- 3细胞依赖 TPO增殖效应的影响评价抗体性质 .酶联免疫法测定血清 rh TPO浓度表明在第 1 ,7和 2 0次注药后 cmax和 AUC0 -τ随注药次数增加明显降低 ,而 Tp 提前 ,提示 TPO存在时间依赖性非线性过程 .血小板于 d5开始增高 ,d 1 4达峰值 ,d 2 0仍保持较高水平 (2 97±2 7) % .于 d 9- 1 1出现低滴度抗 TPO抗体 ,d 2 1达最高滴度 (1∶ 2 5- 1∶ 1 2 5) .sc TPO和白蛋白后猴血清对 TD- 3依赖 TPO增殖效应均有轻微抑制作用 ,抑制曲线呈非中和抗体性质 .
To study the pharmacokinetics, peripheral platelet count and serum TPO antibody changes of rhesus macaques after repeated intravenous administration of rh TPO 2 μg · kg-1 · d-1 × 20 d or albumin, The effect of serum on the proliferation of TD-3 cells dependent on TPO was evaluated by evaluating the antibody properties.Serum rh TPO concentration measured by enzyme-linked immunosorbent assay showed that the cmax and AUC0 -τ significantly decreased with the increase of injection times after the first, seventh and 20th injection , While Tp advanced, suggesting that there is a time-dependent non-linear process of TPO platelets began to increase in the d5, d 1 4 peak, d 2 0 remained high level (2 97 ± 2 7)% in d 9-1 1 Low titer anti-TPO antibody showed the highest titer of d 2 1 (1: 2 5: 1: 1: 1 5) .The sc TPO and albumin post-monkey serum significantly inhibited the TD-3 dependent TPO proliferation. Inhibitory curves showed non-neutralizing antibody properties.