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目的:探讨黄精多糖治疗帕金森病(Parkinson disease,PD)大鼠的疗效和作用机理。方法:建立帕金森病模型,随机分成4组:正常对照组(CG)、模型组(MG)、低剂量治疗组(LDG)和高剂量治疗组(HDG),每组12只。CG组和MG组予以等量注射用水,LDG组和HDG组分别予以4%和16%的黄精多糖溶液灌胃,连续6周。8周时分别观察各组阿扑吗啡诱导的旋转行为的变化,western检测过氧化物酶体增殖物激活受体-γ(peroxisome proliferator-activated receptor-γ,PPAR-γ)蛋白表达,组织化学方法检测酪氨酸羟化酶(tyrosin hydroxylase,TH)表达并计数阳性细胞数。结果:治疗8周后,LDG组和HDG组旋转次数均明显下降(P<0.05),PPAR-γ蛋白表达上调,与MG比较,差异具有显著性(P<0.01)。同时,LDG组和HDG组TH的表达均上调,TH阳性细胞数增多,与MG组比较,差异具有显著性(P<0.01)。结论:黄精多糖具有明显改善PD大鼠行为的作用,机制可能与黄精多糖上调PPAR-γ表达相关,进而抑制炎症反应和细胞凋亡,促进多巴胺神经元的再生。
Objective: To investigate the therapeutic effect and mechanism of Polygonatum Polysaccharide on Parkinson disease (PD) rats. Methods: The Parkinson’s disease model was established and randomly divided into 4 groups: normal control group (CG), model group (MG), low dose treatment group (LDG) and high dose treatment group (HDG), 12 in each group. The rats in CG group and MG group were given equal volume of water for injection. The LDG group and HDG group were given gavage with 4% and 16% polygalacturonase respectively for 6 weeks. At 8 weeks, the changes of apomorphine-induced rotational behavior were observed in each group. The expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) The tyrosine hydroxylase (TH) expression was detected and the number of positive cells was counted. RESULTS: After 8 weeks of treatment, the number of rotations in LDG group and HDG group were significantly decreased (P <0.05), and the expression of PPAR-γ protein was up-regulated. Compared with MG group, the difference was significant (P <0.01). At the same time, the expression of TH in LDG group and HDG group were all increased, and the number of TH positive cells increased. Compared with MG group, the difference was significant (P <0.01). CONCLUSION: Polygonatum polysaccharide can significantly improve the behavior of PD rats. The mechanism may be related to the up-regulation of PPAR-γ expression by Polygonatum Polysaccharides, which can inhibit the inflammatory reaction and apoptosis and promote the regeneration of dopamine neurons.