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本研究选用旱稻、R974//R974/东乡野生稻回交重组自交系及其亲本等66份水稻材料,进行苗期抗旱性鉴定及其综合指标筛选,测定了水分胁迫下最大根长、根基数、茎长、根干重、根鲜重、根系相对含水量、叶片相对含水量、卷叶级别及干旱反复存活率等9个性状。利用主成分分析和逐步回归分析法进行苗期抗旱综合评价,结果表明株系1949最为抗旱;回归分析和相关分析表明最大根长、根数、根鲜重和根系相对含水量对抗旱性影响显著,可作为苗期抗旱性鉴定综合指标。采用综合抗旱D值进行抗旱遗传分析,表明BILs群体的苗期抗旱性表现2对独立主基因+多基因控制。结果可知,东乡野生稻可作为水稻抗旱遗传改良的宝贵资源。
In this study, 66 rice cultivars including upland rice, R974 / R974 / Dongxiang wild rice backcross recombination inbred lines and their parents were selected for drought resistance identification and comprehensive index screening. The maximum root length, Number of stems, root length, root dry weight, root fresh weight, root relative water content, leaf relative water content, leaf curl level and drought survival rate. The results of principal component analysis and stepwise regression analysis showed that strain 1949 was the most drought-tolerant. Regression analysis and correlation analysis showed that the maximum root length, root number, root fresh weight and root relative water content had a significant effect on drought resistance , Can be used as seedling drought resistance identification comprehensive index. Drought resistance genetic analysis using integrated drought resistance D values indicated that the seedling drought tolerance of BILs population was controlled by two independent major genes plus multiple genes. The results showed that Dongxiang wild rice can be used as a valuable resource for rice drought genetic improvement.