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通过Na BH4还原Ag NO3得到胶体银纳米粒子,制作了以该纳米粒子修饰的银电极,研究了其在电催化中的应用,并对相关机理进行了探讨。基于酪氨酸对纳米银的还原信号有明显抑制作用,建立了胶体银纳米粒子修饰银电极在Na Ac-HAc缓冲溶液中用差分脉冲法检测酪氨酸的方法,并讨论了优化工作条件。结果表明,在p H=5.5时,峰电流与酪氨酸的浓度在1.0×10-8~1.0×10-3mol/L范围内呈良好的线性关系,检出限为4.2×10-9mol/L,峰电流Ip与酪氨酸浓度的负对数p C的线性回归方程为Ip(μA)=7.64 p C-15.69(R=99.73%)。用该方法检测氨基酸注射液中酪氨酸的含量,加标回收率在95.2%~107.8%之间。
The colloidal silver nanoparticles were obtained by the reduction of AgNO3 with Na BH4, and the silver electrodes modified with the nanoparticles were prepared. Their application in electrocatalysis was studied, and the related mechanism was also discussed. Based on tyrosine’s inhibition of the reduction signal of nano-silver, a method for detecting tyrosine by differential pulse method in colloidal silver nanoparticle modified silver electrode in Na Ac-HAc buffer solution was established and the optimized working conditions were discussed. The results showed that there was a good linear relationship between the peak current and the concentration of tyrosine in the range of 1.0 × 10-8 ~ 1.0 × 10-3mol / L at pH = 5.5 with the detection limit of 4.2 × 10-9mol / L, the linear regression equation of negative logarithm p C of peak current Ip and tyrosine concentration is Ip (μA) = 7.64 p C -15.69 (R = 99.73%). The content of tyrosine in amino acid injection was detected by this method, and the recoveries were between 95.2% and 107.8%.