目的　探讨恒河猴实验感染新型无包膜DNA病毒 (TTV)的组织嗜性。方法　实验感染的恒河猴在病毒血症期间杀死 5只 ,取组织 ,用聚合酶链反应 (PCR)产物进行病毒半定量 ,并用原位杂交、负义探针斑点杂交和杂交 /核酸保护试验 ,检查复制型病毒DNA分子。结果　肝、外周血单个核细胞 (PBMC)和血清病毒含量 (电泳带密度 )依次为 5 0 1%、31 0 %和 18 9% ,相对比为 2 6 5∶1 6 4∶1 0 0。肝、脾、胃、小肠各段及其淋巴结、PBMC、结肠和血清均可检出病毒 ,但正链病毒DNA(假定的病毒复制中间体 ) ,只稳定存在于肝、脾、小肠和淋巴样细胞中。结论　这一病毒具肝、小肠和淋巴样细胞嗜性 ,可解释其多途径传播。 Objective To investigate the tissue tropism of a novel non-enveloped DNA virus (TTV) infected by rhesus monkeys. Methods Experimentally infected rhesus monkeys were killed 5 during viremia and tissues were harvested for semi-quantification of the virus using polymerase chain reaction (PCR) products and probed with in situ hybridization, negative-sense probe dot blot hybridization and hybridization / nucleic acid protection Test, check for replicative viral DNA molecules. Results The contents of liver, peripheral blood mononuclear cells (PBMCs) and serum viruses (electrophoretic band density) were 501, 31 0% and 18 9%, respectively, with a relative ratio of 26.5:1.6 4:100. The virus was detectable in the liver, spleen, stomach, small intestine and its lymph nodes, PBMC, colon and serum, but the positive strand virus DNA (hypothetical viral replication intermediate) was only stably present in the liver, spleen, small intestine and lymphoid Cells. Conclusion The virus has liver, small intestine and lymphoid cell tropism, which explains its multi-channel transmission.