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目的:研究可溶性IL-15Rα与脾细胞共同孵育后对小鼠黑色素瘤细胞生长的免疫调节作用。方法:制备小鼠脾细胞,分成两组,一组加入可溶性重组IL-15Rα(sIL-15Rα),另外一组不加,培养48 h后,分离两组的贴壁细胞和非贴壁细胞,流式细胞术(FCM)分析CD4、CD8、B220、CD11c、CD1a的表达;并把上述4组细胞(即脾细胞非贴壁细胞组,脾细胞贴壁细胞组,脾细胞+sIL-15Rα非贴壁细胞组,脾细胞+sIL-15Rα贴壁细胞组)和黑色素瘤细胞一起分别注射到小鼠腹部皮下,观察小鼠腹部皮下肿瘤生长抑制情况,对照组只注射小鼠黑色素瘤细胞。结果:脾细胞+sIL-15Rα贴壁细胞组小鼠黑色素瘤的生长速度显著小于其他各组;FCM分析,此组细胞主要成分可能为树突状细胞(DC)。结论:sIL-15Rα与脾细胞共同孵育后,可能通过DC的作用,对黑色素瘤的生长进行免疫调节,从而抑制瘤细胞的生长。
Objective: To study the immunomodulatory effect of soluble IL-15Rα and spleen cells on the growth of mouse melanoma cells. Methods: The spleen cells were prepared and divided into two groups. One group was added with soluble recombinant IL-15Rα (sIL-15Rα), the other group was not added. After cultured for 48 hours, adherent cells and non-adherent cells were separated, The expression of CD4, CD8, B220, CD11c and CD1a was analyzed by flow cytometry (FCM). The above 4 groups of cells (ie spleen nonadherent cell group, splenocyte adherent cell group, splenocyte + sIL- Adherent cells, splenocytes + sIL-15Rα adherent cells) and melanoma cells were injected into the abdomen of mice subcutaneously to observe the growth of mouse subcutaneous tumor growth inhibition, the control group was injected only mouse melanoma cells. Results: The growth rate of melanoma in splenocytes + sIL-15Rα adherent cells was significantly lower than that in other groups. The main components of this group of cells may be dendritic cells (DCs). CONCLUSIONS: When sIL-15Rα is incubated with splenocytes, the growth of melanoma may be immunoregulated through the action of DC, which may inhibit the growth of tumor cells.