论文部分内容阅读
目的探讨细胞毒性T淋巴细胞抗原4(CTLA-4)基因第一外显子49位点A/G基因多态性与原因不明复发性流产(URSA)发病的相关性。方法采用PCR限制性片断长度多态性方法(PCR-RFLP),检测168例URSA患者(URSA组)和117例有正常生育史的妇女(对照组)CTLA-4基因第一外显子49位点A/G多态性,并比较等位基因G/A、基因型AA/AG/GG、表型A+(AA+AG)/G+(GG+AG)分布频率的差异。结果URSA组等位基因G的出现频率为68·4%(230/336),对照组为59·4%(139/234),两组比较,差异有统计学意义(P<0·05);基因型GG的出现频率URSA组为48·8%(82/168),对照组为33·3%(39/117),两组比较,差异也有统计学意义(P<0·05);URSA组基因型AG、基因表型A+(AA+AG)的频率分别为39·3%(66/168)、51·2%(86/168),对照组分别为52·1%(61/117)、66·7%(78/117),两组比较,差异均有统计学意义(P<0·05)。结论CTLA-4基因第一外显子49位点A/G多态性与URSA的发生存在相关性,并可能参与流产发生的免疫病理过程。
Objective To investigate the association between polymorphism of 49 locus A / G gene of cytotoxic T lymphocyte antigen 4 (CTLA-4) gene and the incidence of recurrent spontaneous abortion (URSA) of unexplained origin. Methods PCR-RFLP was used to detect the polymorphisms of CTLA-4 gene exon 49 in 168 URSA patients (URSA group) and 117 women with normal fertility history (control group) Point A / G polymorphism and to compare the distribution frequency of allele G / A, genotype AA / AG / GG, phenotype A + (AA + AG) / G + (GG + AG) Results The frequency of allele G in URSA group was 68.4% (230/336) in control group and 59.4% (139/234) in control group. The difference was statistically significant (P <0.05) The frequency of genotype GG was 48.8% (82/168) in URSA group and 33.3% (39/117) in control group. There was also significant difference between the two groups (P <0.05). The frequencies of genotype AG and genotype A + (AA + AG) in URSA group were 39.3% (66/168) and 51.2% (86/168) respectively, while those in control group were 52.1% (61 / 117) and 66.7% (78/117), respectively. There was significant difference between the two groups (P <0.05). Conclusions A / G polymorphism of CTLA-4 gene exon 49 is associated with the occurrence of URSA and may be involved in the immunopathological process of abortion.