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玉米不仅是一种主要的粮食作物,而且是重要的饲料和工业原料。但是,杂草的威胁导致其产量和质量的急剧下降。草甘膦作为农达除草剂的有效成分,通过抑制植物体内的5烯醇式丙酮莽草酸-3-磷酸合成酶(EPSPS)活来阻止芳香族氨基酸的合成以达到除草目的,其中EPSPS酶是由aroA基因编码的。本实验从荧光假单胞菌(Pseudomonas fluorescens)菌株G2中鉴别出新的突变aroA基因——G2-aroA基因,编码高度抗草甘膦的EPSPS。将G2-aroA基因转入玉米基因组中,赋予其耐草甘膦的特性,对转G2-aroA基因的耐草甘膦玉米进行为期90 d的Sprague-Dawley(SD)大鼠(Rattus norvegicus)喂养实验以评估其食用安
Corn is not only a major food crop but also an important feed and industrial raw material. However, the threat of weeds has led to a sharp decline in its production and quality. Glyphosate, as the active ingredient of Nongda herbicide, prevents the synthesis of aromatic amino acids by inhibiting the production of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in plants in order to achieve the purpose of weeding. EPSPS enzyme is Encoded by the aroA gene. In this experiment, a new mutant aroA gene, G2-aroA gene, was identified from Pseudomonas fluorescens strain G2 and encodes a highly glyphosate-resistant EPSPS. The G2-aroA gene was introduced into the maize genome to confer glyphosate-tolerant properties. The glyphosate-tolerant maize transformed with the G2-aroA gene was fed with 90-day-old Sprague-Dawley (SD) rats (Rattus norvegicus) Experiment to assess their food safety