论文部分内容阅读
目的:Toll样受体9(Toll-like receptor-9,TLR9)是天然免疫受体之一,近期研究表明TLR9与多种上皮性肿瘤发生的早期行为密切相关。本研究拟从蛋白质水平观察宫颈上皮组织中TLR9表达变化与维吾尔族妇女宫颈癌病理进程的关系。方法:收集维吾尔族妇女宫颈炎、宫颈上皮内瘤变cervical intraepithelial neoplasia,CIN)和宫颈鳞癌(cervical squamous cell carcinoma,CSCC)患者的宫颈上皮石蜡包埋组织标本共97例,采用免疫组织化学方法检测各组织中TLR9蛋白表达水平。结果:TLR9蛋白在绝大部分宫颈炎患者的宫颈上皮细胞中不表达(88.2%)或弱表达(11.8%),但是随着宫颈上皮内瘤变和宫颈癌的发生,表达水平逐渐上升,其阳性率依次为慢性宫颈炎(2/17,11.8%),CINⅠ(4/19,28.6%),CINⅡ(3/10,30.0%),CINⅢ(12/24,50.0%),CSCC(17/32,53.1%),5组之间比较差异有统计学意义(P<0.05),CINⅢ及CSCC与宫颈炎间的阳性率差异显著(P<0.05),但是CINⅠ和CINⅡ与宫颈炎间的差异无统计学意义(P>0.05),并且在CSCC中,不同肿瘤分化程度之间其TLR9蛋白阳性率的差异无统计学意义(P>0.05)。结论:TLR9蛋白可能参与了宫颈癌的发生和发展的进程。
AIM: Toll-like receptor-9 (TLR9) is one of the innate immune receptors. Recent studies have shown that TLR9 is closely related to the early behavior of various epithelial tumors. This study was to observe the relationship between TLR9 expression in cervical epithelial tissue and the pathological progress of cervical cancer in Uighur women at the protein level. Methods: A total of 97 specimens of cervical epithelial paraffin embedded in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC) were collected from Uighur women. Immunohistochemistry Tissue TLR9 protein expression levels were detected. Results: TLR9 protein was not expressed (88.2%) or weakly expressed (11.8%) in cervical epithelial cells in the majority of cervicitis patients. However, with the development of cervical intraepithelial neoplasia and cervical cancer, the expression level of TLR9 protein increased The positive rates were as follows: chronic cervicitis (2/17, 11.8%), CINⅠ (4/19, 28.6%), CINⅡ (3/10, 30.0%), CINⅢ 32,53.1%). The difference between the 5 groups was statistically significant (P <0.05). The positive rates of CINⅢ, CSCC and cervicitis were significantly different (P <0.05). However, the differences between CINⅠ and CINⅡ and cervicitis (P> 0.05). There was no significant difference in the positive rate of TLR9 protein between CSCC and CSCC (P> 0.05). Conclusion: TLR9 protein may be involved in the occurrence and development of cervical cancer.