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目的探讨细胞周期蛋白A(CyclinA)及P21在子宫内膜异位症发生、发展中的作用。方法采集子宫内膜异位症患者(观察组)在位内膜(25例)和异位子宫内膜(28例)以及其他良性疾病患者的子宫内膜(对照组35例),用免疫印迹、RT-PCR、免疫组化的方法检测CyclinA和P21表达。结果cyclinAmRNA在异位内膜增生期与分泌期表达量(1.24±0.10)、(1.33±0.21)均明显高于在位内膜(0.72±0.26)、(0.42±0.22)及对照组(0.68±0.09)、(0.35±0.06)(P<0.05)。CyclinA蛋白在异位内膜增生期与分泌期表达量(0.95±0.10)、(0.91±0.18)均明显高于在位内膜(0.53±0.08)、(0.27±0.11)及对照组(0.47±0.11)、(0.22±0.09)(P<0.05)。在位内膜CyclinA表达量与对照组相比均无显著性差异(P>0.05)。P21mRNA在对照组内膜增生期与分泌期表达量(0.21±0.01)、(0.54±0.35)均明显高于在位内膜(0.09±0.06)、(0.28±0.02)及异位内膜(0.06±0.01)、(0.13±0.00)(P<0.05)。P21蛋白在对照组内膜增生期与分泌期表达量(0.74±0.13)、(1.13±0.15)均明显高于在位内膜(0.59±0.21)、(0.62±0.29)及异位内膜(0.40±0.36)、(0.43±0.33)(P<0.05)。CyclinA在对照组内膜增生期表达高于分泌期(P<0.05),P21在对照组内膜分泌期表达高于增生期(P<0.05),而两者在异位内膜表达均失去周期性(P>0.05),两者在异位内膜中表达呈显著负相关(r=-0.738,P<0.01)。结论CyclinA及P21异常表达可能在子宫内膜异位症异位内膜增殖中起重要作用。
Objective To investigate the role of Cyclin A and P21 in the pathogenesis and progression of endometriosis. Methods The endometrium (25 cases) and ectopic endometrium (28 cases) and the endometrium of other benign diseases (control group) were collected from patients with endometriosis (observation group) , RT-PCR, immunohistochemical methods to detect the expression of CyclinA and P21. Results The expression of cyclinA mRNA in ectopic endometrium was significantly higher than that in eutopic endometrium (1.24 ± 0.10, 1.33 ± 0.21, 0.42 ± 0.26, 0.68 ± 0.22, 0.09), (0.35 ± 0.06) (P <0.05). The expression of CyclinA protein in ectopic endometrium was 0.95 ± 0.10 and 0.91 ± 0.18 respectively, which was significantly higher than that in eutopic endometrium (0.27 ± 0.11) and control group (0.47 ± 0.11) 0.11), (0.22 ± 0.09) (P <0.05). The expression of CyclinA in eutopic endometrium was not significantly different from that in control group (P> 0.05). The expression of P21mRNA in the control group was significantly higher than that in eutopic endometrium (0.21 ± 0.01), (0.54 ± 0.35), (0.28 ± 0.02) and ectopic endometrium ± 0.01), (0.13 ± 0.00) (P <0.05). The expression of P21 protein in the proliferative phase and secretory phase in the control group was significantly higher than that in the eutopic endometrium (0.92 ± 0.21), (0.62 ± 0.29) and ectopic endometrium 0.40 ± 0.36), (0.43 ± 0.33) (P <0.05). The expression of CyclinA in the control group was higher than that in the secretory phase (P <0.05), P21 was higher in the endometrium during the secretory phase (P <0.05), while the expression of CyclinA in the control group was no longer than that in the secretory phase (P> 0.05). There was a significant negative correlation between the two expressions in ectopic endometrium (r = -0.738, P <0.01). Conclusion The abnormal expression of CyclinA and P21 may play an important role in the ectopic endometrial proliferation of endometriosis.