论文部分内容阅读
目的观察健择致胰腺癌细胞株蛋白质变化的影响,寻找一些在化疗过程中出现的蛋白质, 为临床治疗提供理论依据。方法利用蛋白质组学技术,分析胰腺癌细胞株SW-1990在加入健择和5- FU后其蛋白质谱的变化,最后鉴定其差异表达的蛋白质。结果培养细胞受抑制约50%时,健择为1- 100 ng/ml,5-FU为250-2 500 ng/ml。质谱鉴定了9个差异表达的蛋白质,其中对照组3个,加药组6个。结论健择对细胞株的作用明显优于5-FU。β-肌动蛋白、MGC:19713等某些高表达的蛋白质可能成为观察化疗效果的重要指标。
Objective To observe the effect of protein changes in pancreatic cancer cell lines of Jian-e select some protein in the process of chemotherapy to provide a theoretical basis for clinical treatment. Methods Proteomic analysis of pancreatic cancer cell line SW-1990 was carried out to analyze the changes of protein profile after addition of gemcitabine and 5-FU. Finally, the differentially expressed proteins were identified. Results When the cultured cells were inhibited by about 50%, the optimal concentration was 1- 100 ng / ml and the 5-FU was 250-2 500 ng / ml. Mass spectrometry identified 9 differentially expressed proteins, including 3 in the control group and 6 in the dosing group. Conclusion The effect of gemcitabine on cell lines is obviously better than that of 5-FU. β-actin, MGC: 19713 and some other highly expressed proteins may become an important indicator of the effect of chemotherapy.