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目的研究蛛网膜下腔出血(SAH)后血管内皮细胞生长因子C(VEGF-C)在心、肺的表达及重组人干扰素-β(INF-β)对心肺功能的保护作用。方法采用小脑延髓池注血法建立蛛网膜下腔出血模型,将实验动物随机分为正常对照组、假手术组、SAH未治疗组和SAH治疗组,采用免疫组织化学SP法观察VEGF-C在心脏、肺脏中的表达并测定积分光密度值(IOD)。结果与假手术组相比,SAH未治疗组和SAH治疗组,心脏和肺脏中VEGF-C的表达在24、48、72 h时均明显升高(P均<0.05);SAH治疗组24、48、72 h时,VEGF-C在心脏和肺脏中的表达均低于SAH未治疗组(P均<0.05)。结论 SAH引起的全身炎症反应导致心、肺缺血,使VEGF-C的表达升高,INF-β可抑制心、肺等脏器的炎症反应并改善组织缺血。
Objective To investigate the expression of vascular endothelial growth factor C (VEGF-C) in the heart and lung after subarachnoid hemorrhage (SAH) and the protective effect of recombinant human interferon-β (INF-β) on cardiopulmonary function. Methods The subarachnoid hemorrhage model was established by the cerebellar cistern injection. The experimental animals were randomly divided into normal control group, sham operation group, SAH untreated group and SAH treatment group. Immunohistochemical SP method was used to observe the expression of VEGF-C Heart, lung, and determine the integrated optical density (IOD) value. Results Compared with sham operation group, the expression of VEGF-C in SAH untreated group and SAH treated group, heart and lung increased significantly at 24, 48 and 72 h (all P <0.05). In SAH treated group, At 48 and 72 h, the expression of VEGF-C in heart and lung was lower than that in SAH untreated group (all P <0.05). Conclusions Systemic inflammatory response induced by SAH leads to cardiac and pulmonary ischemia, which leads to the increase of VEGF-C expression. INF-β can inhibit the inflammatory reaction in heart and lung and improve the tissue ischemia.