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目的检测骨科患者感染鲍曼不动杆菌16S rRNA基因分布情况,分析菌株耐药性,为有效的临床治疗提供指导。方法收集骨科患者临床送检标本,分离并鉴定鲍曼不动杆菌,PCR法进行菌株16S rRNA基因的检测,K-B法分析菌株耐药性。结果 48株鲍曼不动杆菌临床分离株对氨基糖苷类抗菌药物阿米卡星、链霉素、庆大霉素、妥布霉素、卡那霉素的耐药率分别为12.50%、56.25%、43.75%、47.92%和27.08%,对链霉素的耐药率最高。对鲍曼不动杆菌的armA基因、rmtA基因、rmtB基因、rmtC基因、rmtD基因以及nmpA基因均进行了检测,仅检出armA基因。armA基因片段大小为590bp,且该基因阳性率为100.00%。结论骨科患者感染鲍曼不动杆菌对常用氨基糖苷类药物产生了一定程度的耐药性,armA的存在可能与菌株耐药性产生存在关系。
Objective To detect the distribution of 16S rRNA gene of Acinetobacter baumannii in orthopedic patients and analyze the drug resistance of the strains to provide guidance for effective clinical treatment. Methods Clinical specimens of orthopedic patients were collected, Acinetobacter baumannii was isolated and identified. The 16S rRNA gene was detected by PCR, and the drug resistance of strains was analyzed by K-B method. Results The drug resistance rates of 48 clinical isolates of Acinetobacter baumannii to aminoglycoside antibiotics amikacin, streptomycin, gentamicin, tobramycin and kanamycin were 12.50% and 56.25 %, 43.75%, 47.92% and 27.08%, the highest rate of resistance to streptomycin. The armA gene, the rmtA gene, the rmtB gene, the rmtC gene, the rmtD gene and the nmpA gene of Acinetobacter baumannii were detected and only the armA gene was detected. The size of armA gene fragment was 590bp, and the positive rate of this gene was 100.00%. Conclusion Acinetobacter baumannii infection to some commonly used aminoglycosides has some degree of resistance. The existence of armA may be related to the drug resistance of the strains.