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目的研究D-柠檬烯对人胃癌MGC803细胞生长抑制和凋亡的影响。方法噻唑蓝(MTT)法比色检测细胞生长情况;流式细胞仪检测细胞凋亡率;激光共聚焦检测细胞内活性氧(reactive oxygen species,ROS)的含量和caspase- 3蛋白的表达。结果D-柠檬烯对人胃癌MGC803细胞生长抑制作用呈剂量依赖关系;流式细胞仪检测发现D-柠檬烯可引起MGC803细胞凋亡,且呈时间依赖性;激光共聚焦显微镜荧光检测发现D-柠檬烯处理的细胞内ROS明显升高(P<0.05),caspase-3蛋白表达明显增加(P<0.05)。结论D-柠檬烯能够有效地抑制癌细胞的生长以及诱导凋亡。这种作用机制可能是使MGC803细胞内产生大量的活性氧。也可能与细胞内caspase-3基因的表达变化有关。
Objective To study the effects of D-limonene on the growth inhibition and apoptosis of human gastric cancer MGC803 cells. Methods MTT assay was used to detect the cell growth. The apoptosis rate was detected by flow cytometry. The content of reactive oxygen species (ROS) and the expression of caspase-3 protein were detected by confocal laser scanning microscopy. Results D-limonene inhibited the growth of MGC803 cells in a dose-dependent manner. Flow cytometry revealed that D-limonene could induce apoptosis in MGC803 cells in a time-dependent manner. Fluorescence confocal microscopy revealed that D-limonene (P <0.05), while the expression of caspase-3 protein increased significantly (P <0.05). Conclusion D-limonene can effectively inhibit the growth of cancer cells and induce apoptosis. This mechanism may be MGC803 cells produce large amounts of reactive oxygen species. It may also be related to the change of intracellular caspase-3 gene expression.