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目的:探讨三七总皂苷(Panax notoginseng Total Saponins)对D-半乳糖致心肌细胞株H9c2细胞衰老的保护作用及其可能机制。方法:50 mmol/L D-半乳糖处理H9c2细胞建立衰老模型。不同浓度三七总皂苷(5、25、50μg/mL)预处理细胞4 h,再加入D-半乳糖继续培养24 h。β-半乳糖苷酶染色法鉴定细胞衰老程度,DCFH-DA探针检测细胞内活性氧(Reactive Oxygen species,ROS)水平,生化法检测超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,Hochest染色分析细胞凋亡情况。结果:与正常对照组比较,D-半乳糖组β-半乳糖苷酶染色阳性细胞数目显著增多,ROS荧光强度显著上升,SOD活性降低,MDA含量增加,Hochest染色发现细胞核染色质浓缩和凝聚,并可见大量凋亡小体;与D-半乳糖组比较,不同浓度三七总皂苷组β-半乳糖苷酶染色阳性细胞数目显著减少,SOD活性显著升高,MDA含量显著降低,ROS荧光强度显著降低,细胞核染色质浓缩和凝聚以及凋亡小体均有所改善。结论:三七总皂苷可通过提高抗氧化能力和减少细胞凋亡来对抗D-半乳糖所致的H9c2细胞衰老。
Objective: To investigate the protective effect of Panax notoginseng Total Saponins on senescence of H9c2 cells induced by D-galactose and its possible mechanism. Methods: Aging models were established by treating H9c2 cells with 50 mmol / L D-galactose. The cells were pretreated with different concentrations of Panax notoginseng saponins (5, 25 and 50 μg / mL) for 4 h and then cultured for 24 h with D-galactose. The degree of cell senescence was identified by β-galactosidase staining, the level of reactive oxygen species (ROS) was detected by DCFH-DA probe and the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) ) Content, Hochest staining analysis of apoptosis. Results: Compared with the normal control group, the number of β-galactosidase positive cells in D-galactose group increased significantly, ROS fluorescence intensity increased significantly, SOD activity decreased, MDA content increased, Hochest staining found that the chromatin condensation and aggregation, And a large number of apoptotic bodies were observed. Compared with D-galactose group, the number of β-galactosidase positive cells in different concentrations of Panax notoginseng saponins significantly decreased, the activity of SOD increased significantly, MDA content decreased significantly, ROS fluorescence intensity Significantly decreased, nuclear chromatin condensation and aggregation, and apoptotic bodies were improved. Conclusion: Panax notoginseng saponins can antagonize the aging of H9c2 cells induced by D-galactose by increasing the antioxidant capacity and reducing apoptosis.