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目的比较单纯酵母喂饲和氧嗪酸联合酵母暴露高尿酸血症肾病大鼠模型的抗氧化活力。方法将30只雄性SD大鼠随机分为3组,每组10只,分别为对照组(饲以普通大鼠颗粒饲料)、酵母组(饲以高酵母饲料)和氧嗪酸钾+酵母组(接受酵母组相同处理的同时,采用腹腔注射方式染毒100 mg/kg氧嗪酸钾乳悬液,染毒容量为4 ml/kg,每天1次);连续染毒4周。检测血清尿素氮(BUN)、肌酐(CREA)、尿酸(URIC)、三酰甘油(TG)、总胆固醇(CHOL)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平及超氧化歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和过氧化氢含量。结果与对照组比较,酵母组和氧嗪酸钾+酵母组大鼠血清中BUN、CREA、URIC、HDL-C含量均较高,TG含量均较低,差异有统计学意义(P<0.05)。与酵母组比较,氧嗪酸钾+酵母组大鼠血清中BUN含量较低,CREA含量较高,差异有统计学意义(P<0.05)。各组大鼠血清中CHOL、LDL-C含量间比较,差异无统计学意义。氧嗪酸钾+酵母组和酵母组大鼠血清中SOD活力均低于对照组,差异有统计学意义(P<0.05);而对照组和酵母组大鼠血清中GSH-Px活力均高于氧嗪酸钾+酵母组,差异有统计学意义(P<0.05)。且各组大鼠血清中过氧化氢含量间比较,差异无统计学意义。结论单纯酵母喂饲和氧嗪酸联合酵母两种建模方法都能够建立持续的高尿酸血症肾病大鼠模型,但氧嗪酸联合酵母饲喂建立的高尿酸血症肾病大鼠模型中大鼠抗氧化活力较单纯酵母饲喂建立的模型中的大鼠下降更为显著。
OBJECTIVE: To compare the antioxidant activity of rat model of hyperuricemia nephropathy induced by yeast and oxonic acid combined with yeast. Methods Thirty male Sprague-Dawley rats were randomly divided into three groups (n = 10), which were control group (fed with normal rat pellet), yeast group (fed with high yeast feed) and oxonic acid potassium + yeast group (In the same way as the yeast group, 100 mg / kg oteracil potassium milk suspension was injected intraperitoneally at a dose of 4 ml / kg once a day for the same treatment in the yeast group; Serum levels of BUN, CREA, URIC, TG, CHOL, HDL-C and LDL-C were measured. Level and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and hydrogen peroxide content. Results Compared with the control group, the serum levels of BUN, CREA, URIC and HDL-C in the yeast group and potassium oxonate + yeast group were higher than those in the control group, and the contents of TG were lower (P <0.05) . Compared with the yeast group, the serum levels of BUN and the content of CREA in the otinic acid potassium + yeast group were lower, and the difference was statistically significant (P <0.05). The levels of CHOL and LDL-C in the serum of the rats in each group showed no significant difference. Oxyzine potassium + yeast group and yeast group serum SOD activity were lower than the control group, the difference was statistically significant (P <0.05); while the control group and yeast group serum GSH-Px activity were higher Oxonic acid potassium + yeast group, the difference was statistically significant (P <0.05). There was no significant difference between the two groups in serum hydrogen peroxide content. Conclusion Both simple yeast feeding and oxonic acid combined with yeast modeling methods can establish a sustained hyperuricemic nephropathy rat model, but the oxonic acid combined with yeast fed hyperuricemia rats model established in large The antioxidant activity of the rats decreased more significantly than that in the model established by yeast feeding alone.