论文部分内容阅读
为研究氟醚菌酰胺(N-(3-氯-5-(三氟甲基)吡啶-2-甲基)-2,3,5,6-四氟-4-甲氧基苯甲酰胺)对辣椒疫霉的作用机制,采用生物测定法系统测定了氟醚菌酰胺对辣椒疫霉菌丝生长、游动孢子释放和菌丝生长量的影响,并进一步对药剂处理后的辣椒疫霉菌丝的超微结构进行了观察;同时测定了氟醚菌酰胺对病原菌糖酵解、三羧酸循环和磷酸戊糖3种呼吸代谢途径的影响,以及其对辣椒疫霉菌丝体细胞膜通透性、可溶性蛋白和DNA含量的影响。结果表明:氟醚菌酰胺对辣椒疫霉菌丝生长、游动孢子释放和菌丝生长量的EC50值分别为7.14、16.34和5.12μg/m L;其可使辣椒疫霉菌丝分支增多变短,且出现细胞壁增厚和细胞变形现象;ATP的存在能降低氟醚菌酰胺对辣椒疫霉菌丝的抑制作用,说明氟醚菌酰胺对辣椒疫霉能量产生过程有一定的抑制作用;其对三羧酸循环途径的抑制作用最为明显。电导率法测定结果表明,氟醚菌酰胺处理均能提高辣椒疫霉的细胞膜通透性,用100μg/m L的氟醚菌酰胺处理400 min后,菌株的相对渗率达78.23%。但氟醚菌酰胺对辣椒疫霉生物大分子的影响较小。研究结果初步表明,氟醚菌酰胺有多个作用位点,但主要是通过抑制辣椒疫霉能量产生和细胞膜通透性而起到抑菌作用。
For the study of fluorozone bacteria (N- (3-chloro-5- (trifluoromethyl) pyridin-2-ylmethyl) -2,3,5,6-tetrafluoro-4-methoxybenzamide) The mechanism of action on Phytophthora capsici was investigated by bioassay system. The effects of tetraflufenamid on the growth, zoospore release and mycelium growth of Phytophthora capsici were studied. The effects of fluoroanisolamide on the three pathways of metabolic metabolism of pathogenic bacteria, such as glycolysis, tricarboxylic acid cycle and pentose phosphate, and their effects on the membrane permeability, soluble Effects of protein and DNA content. The results showed that the EC50 values of difluorate for mycelial growth, zoospore release and mycelial growth of Phytophthora capsici were 7.14, 16.34 and 5.12 μg / m L, respectively. And cell wall thickening and cell deformation appeared; the presence of ATP could reduce the inhibitory effect of tetraflufenacil on Phytophthora capsici, indicating that the fluoroether bacteria inhibited the energy production of Phytophthora capsici; Inhibition of acid cycle pathway most obvious. The results of conductivity measurement showed that both of them could increase the cell membrane permeability of Phytophthora capsici, and the relative permeability of the strain reached 78.23% after treatment with 100 μg / mL of tetraflufenamid for 400 min. But tetraflufenamide had less effect on Phytophthora capsici biomacromolecules. The results showed that there were multiple sites of flufenamic acid amide, but mainly inhibited the production of Phytophthora capsici and cell membrane permeability and played an antibacterial effect.