论文部分内容阅读
胚胎植入过程中,滋养层细胞浸润与肿瘤的迁移过程非常相似,但显著的区别在于前者是受严格调控的有节制的浸润,基质金属蛋白酶(MMPs)的许多成员在其中起重要的作用.MMP-26是近年来发现的MMPs家族的新成员,它在滋养层细胞中的作用所知甚少.利用国际常用的人滋养层细胞模型——人绒毛膜上皮癌细胞系(JEG-3)作为体外实验模型,探讨MMP-26在人滋养层细胞浸润调节中的作用.将含有MMP-26全长cDNA的pCR3.1质粒转染到JEG-3细胞中,获得过量表达MMP-26基因的稳定细胞系JEG-3/MMP-26;细胞浸润分析表明JEG/MMP-26细胞的浸润能力较母本细胞明显增强;RT-PCR和明胶酶谱分析显示JEG-3/MMP-26细胞中MMP-9的表达和分泌水平提高;双荧光免疫细胞化学进一步显示MMP-26和MMP-9蛋白在细胞中有共定位现象.上述结果表明MMP-26能有效促进人滋养层细胞浸润,其作用可能是通过与其他MMP分子(如MMP-9)的协调来实现的.
During embryo implantation, the infiltration of trophoblast cells is very similar to that of tumor cells, but the significant difference is that the former is a well-regulated and controlled infiltration of which many members of the matrix metalloproteinases (MMPs) play an important role. MMP-26 is a new member of the MMPs family found in recent years, and its role in trophoblast cells is poorly understood.Using the commonly used human trophoblastic cell model - human choriocarcinoma cell line (JEG-3) As an in vitro experimental model, we explored the role of MMP-26 in the regulation of human trophoblast cell invasion.PCR3.1 plasmid containing the full-length cDNA of MMP-26 was transfected into JEG-3 cells to obtain MMP-26 over-expressed The results of RT-PCR and gelatin zymography showed that the expression of MMP in JEG-3 / MMP-26 cells was significantly higher than that in JEG-3 / MMP-26 cells -9 expression and secretion increased; Dual-fluorescence immunocytochemistry further showed co-localization of MMP-26 and MMP-9 protein in the cells.The above results show that MMP-26 can effectively promote human trophoblast cell invasion, and its role may be Is through association with other MMP molecules (such as MMP-9) Tune to achieve.