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目的观察采用常规降糖治疗与联合N-乙酰半胱氨酸(NAC)治疗对糖尿病慢性肾脏病(CKD)患者多聚ADP核糖聚合酶(PARP)、3-磷酸甘油脱氢酶(GAPDH)活性的影响。方法将60例CKDⅢ期患者随机分为常规治疗组(A组)30例;联合NAC治疗组(B组)30例;另选取同期健康体检者30名为正常对照(NC)组,治疗12周。观察治疗前后外周单核细胞PARP活性、全血GAPDH活性的变化。结果 A、B组治疗后PARP活性下降[A组(3.70±0.06)vs(3.02±0.07)U/mg;B组(3.74±0.11)vs(2.66±0.04)U/mg],GAPDH活性升高[A组(4430.8±279.4)vs(5136.6±321.6)U/ml,B组(4495.2±324.7)vs(6104.4±275.3)U/ml],且B组治疗后PARP及GAPDH活性改变与A组比较,差异有统计学意义(P<0.05)。结论联合NAC治疗与常规治疗相比,可更有效地逆转CKD患者PARP、GAPDH活性的异常。
Objective To observe the effects of conventional hypoglycemic therapy and NAC on the activities of poly ADP-ribose polymerase (PARP), glycerol-3-phosphate dehydrogenase (GAPDH) activity in diabetic patients with chronic kidney disease (CKD) Impact. Methods Thirty patients with CKD stage Ⅲ were randomly divided into routine treatment group (A group) 30 cases, NAC treatment group (B group) 30 cases. Another 30 healthy subjects were selected as normal control group (NC) . The changes of PARP activity and GAPDH activity in peripheral blood mononuclear cells were observed before and after treatment. Results The activities of PARP in group A and group B were decreased (3.70 ± 0.06 vs 3.02 ± 0.07 U / mg in group A and 3.74 ± 0.11 vs 2.66 ± 0.04 U / mg in group B, respectively) [A group (4430.8 ± 279.4 vs 5136.6 ± 321.6 U / ml, B group (4495.2 ± 324.7 vs 6104.4 ± 275.3 U / ml], and the changes of PARP and GAPDH activity after treatment in group B were compared with those in group A , The difference was statistically significant (P <0.05). Conclusion The combination of NAC and conventional therapy can reverse the abnormality of PARP and GAPDH activity more effectively in patients with CKD.