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目的探讨CD4+CD25+high调节性T细胞(Tr)在自身免疫性肝炎(AIH)发病中的作用。方法用流式细胞仪技术比较分析AIH患者16例、慢性乙型肝炎(CHB)22例及键康正常人20例外周血中的CD4+CD25+highTr细胞,同时用免疫组织化学法检测AIH和CHB患者肝组织Foxp3的表达情况。结果AIH组外周血中CD4+CD25+high/CD4+百分比显著低于正常组(P<0.05)和CHB组(P<0.01),并且CHB组显著高于正常组(P<0.05);同时AIH组外周血中CD4+T细胞也显著高于CHB组(P<0.01));肝组织Foxp3+细胞主要分布于肝小叶内窦周隙、汇管区,AIH组肝组织Foxp3+表达显著低于CHB组(P<0.01)。结论CD4+CD25+highTr细胞下降可能是自身免疫性肝炎发病的原因之一。
Objective To investigate the role of CD4 + CD25 + high regulatory T cells (Tr) in the pathogenesis of autoimmune hepatitis (AIH). Methods Flow cytometry was used to compare the levels of CD4 + CD25 + highTr cells in peripheral blood from 16 patients with AIH, 22 with chronic hepatitis B (CHB) and 20 healthy controls. The AIH and Expression of Foxp3 in liver tissue of CHB patients. Results The percentage of CD4 + CD25 + high / CD4 + in peripheral blood of AIH group was significantly lower than that of normal group (P <0.05) and CHB group (P <0.01), and the CHB group was significantly higher than that of normal group (P <0.05) The number of CD4 + T cells in peripheral blood was also significantly higher than that in CHB group (P <0.01). Foxp3 + cells in hepatic tissue were mainly distributed in the perivascular sinus of hepatic lobule. The expression of Foxp3 + in hepatic tissue in portal area and AIH group was significantly lower than that in CHB group <0.01). Conclusion The decrease of CD4 + CD25 + highTr cells may be one of the causes of autoimmune hepatitis.