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采用酶联免疫吸附测定(ELISA)方法,测定玉米苗期接种丝黑穗病菌后植株体内生长素(IAA)、赤霉素(GA)、玉米素核苷(ZR)和脱落酸(ABA)4种内源激素的含量变化,从寄主与病菌互作过程中植物内源激素的变化研究玉米对丝黑穗病的抗性机制。研究结果表明,玉米丝黑穗病菌侵染玉米植株后,植株体内单一激素ABA含量提高;IAA、GA和ZR的含量降低;IAA/ABA、ZR/ABA、GA/ABA及(IAA+GA+ZR)/ABA的比值均明显低于对照。激素间的比值变化较单一激素的含量变化更加显著,相对单一激素的调控作用,激素间相互作用对调节植株生长发育更为重要。玉米丝黑穗病菌侵染玉米的过程中,植物内源激素的一系列变化可能是引起植株出现畸形症状(如节间缩短、植株矮化和丛生等)的直接原因。
The contents of IAA, GA, ZR and ABA 4 in maize seedlings after inoculation with Stem Headwort were determined by enzyme linked immunosorbent assay (ELISA) The changes of endogenous hormones in maize were studied. The mechanism of maize resistance to head smut was studied from the changes of plant endogenous hormones during the process of host-pathogen interaction. The results showed that ABA, IAA, GA and ZR contents of IAA, GA and ZR were decreased in maize plants after infection by corn ear head smut. The content of IAA, GA, ABA and IAA + GA + ZR ) / ABA ratio were significantly lower than the control. Hormone ratio changes more than a single hormone content changes more pronounced, relative to a single hormone regulation, hormone interaction is more important for the regulation of plant growth and development. During the process of maize head smut infection on maize, a series of changes of plant endogenous hormones may be the direct cause of abnormal symptoms (such as internode shortening, plant dwarfing and tufting).