论文部分内容阅读
目的 了解结核分枝杆菌链霉素(SM)耐药基因突变情况,建立快速检测耐药突变株的方法。方法 通过PCR-RFLP分析62株结核分枝杆菌临床分离株的rpsL基因突变的部位和性质。结果 以H37RV标准株为对照,13株敏感株的rpsL基因有MboⅡ酶切位点;37株耐SM分离株中,31株(838%)无MboⅡ酶切位点;12株耐其它抗结核药物株中,也有2株无MboⅡ酶切位点。结论 大多数结核分枝杆菌SM耐药分离株的核糖体S12蛋白编码基因(rpsL)第43位密码子有点突变。PCR-RFLP可能成为快速检测结核分枝杆菌耐药突变株的一种新方法。
Objective To understand the mutation of Mycobacterium tuberculosis streptomycin (SM) resistance gene and to establish a rapid detection method of drug-resistant mutants. Methods The location and nature of rpsL gene mutation in 62 clinical isolates of Mycobacterium tuberculosis were analyzed by PCR-RFLP. Results The H37RV standard strain was used as control. The rpsL gene of 13 susceptible strains had MboⅡ restriction sites. Among the 37 SM-resistant isolates, 31 (838%) isolates did not have MboⅡ restriction sites and 12 were resistant to other anti-tuberculosis drugs There were also 2 non-MboⅡ restriction sites in the strain. Conclusion The codon 43 of ribosomal S12 protein coding gene (rpsL) of most Mycobacterium tuberculosis SM isolates is slightly mutated. PCR-RFLP may become a new method for rapid detection of M. tuberculosis-resistant mutants.