论文部分内容阅读
目的:研究急性心肌梗死(AMI)患者骨髓间充质干细胞(MSC)生物学特性的改变。方法:取AMI患者和对照组骨髓的贴壁细胞,测定其生长曲线和倍增时间,流式细胞仪检测其免疫表型,进行纤维母细胞集落形成单位(CFUF)计数,体外诱导成脂肪和成骨,以油红O及VonKossa染色证实。结果:这些细胞呈梭形贴壁生长,CD105、CD44、CD29、Flk1均阳性,AMI组细胞倍增时间长于对照组[(61.2±4.6)∶(55.1±5.2)h],CFUF低于对照组[(10.2±1.9)∶(13.3±2.9),P<0.05],2组MSC细胞均可成脂肪、成骨。结论:AMI组MSC增殖能力较对照组弱,MSC的异常可能是AMI患者易患动脉粥样硬化原因之一。
Objective: To study the biological characteristics of bone marrow mesenchymal stem cells (MSC) in patients with acute myocardial infarction (AMI). Methods: The adherent cells of bone marrow from AMI patients and control group were measured. The growth curve and doubling time were measured. The immunophenotypes were detected by flow cytometry. The number of fibroblast colony forming units (CFUF) Bone, confirmed with Oil Red O and VonKossa staining. Results: The cells grew in spindle shape with positive staining of CD105, CD44, CD29 and Flk1. The doubling time of AMI group was longer than that of control group [(61.2 ± 4.6) vs (55.1 ± 5.2) h] and CFUF was lower than that of control group [ (10.2 ± 1.9) :( 13.3 ± 2.9), P <0.05]. Both MSC cells could be adipogenic and osteogenic. Conclusion: The proliferation of MSC in AMI group is weaker than that in control group. The abnormality of MSC may be one of the causes of atherosclerosis in AMI patients.